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Histamine detection into crayfish CNS

Using STAINperfect immunostaining kit A, Histamine was detected into the CNS of a crayfish. Staining was obtained following the protocol for whole mounts samples with IS1039 rabbit polyclonal anti-histamine antibody. Fluorescent labeled secondary antibody was used and picture was acquired by confocal imaging.

Histamine antibody - Rabbit polyclonal Ab

Ref: IS1039

 

Histamine is  a widely present natural amino acid known to be involved in different systems including in the control of central nervous and immune functions. The anti-histamine antibody IS1039 is a rabbit polyclonal antibody developped to detect Histamine in various types of samples including whole mounts, cell culture and tissue sections. Importantly, samples must be prepared according to the STAINperfect immunostaining kit A and following the recommended procedure.

 

Clonality Polyclonal antibody
Host Rabbit
Species reactivity Reacts with all species
Tested samples whole mounts, cell culture, tissue sections
Staining procedure STAINperfect immunostaining kit A
Format 50µl (approx. 40 tissue sections)
440
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Product information Protocols References Technical support Reviews

Product overview

 


 

Product name

Histamine antibody - Rabbit pAb

Synonyms

Anti-Histamine antibody
Anti-2-(4-Imidazolyl)ethylamine antibody

Immunogen Conjugated Histamine
Specificity When tested in competitive ELISA, the anti-conjugated Histamine antibody did not show any significant cross reactivity with Histidine conjugate.
Volume 50µL
Lot number 160101

 

 

Storage

 


 

Form

Liquid

Purity Purified anti-serum
Storage

Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.

Material safety datasheet Download MSDS
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Protocols

IF - Cell cultures, Whole mounts, Tissue sections

Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample

Comments

Optimal working dilutions must be determined by the end-user

Restrictions For research use only
Full protocol Download STAINperfect protocol for histamine staining
Protocols-at-a-glance

 

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Complete 
Instructions for Use
Protocol-at-a-glance 
for cell cultures
Protocol-at-a-glance 
for whole mounts
Protocol-at-a-glance 
for tissue sections


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References

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