Histamine detection into crayfish CNS
Using STAINperfect immunostaining kit A, Histamine was detected into the CNS of a crayfish. Staining was obtained following the protocol for whole mounts samples with IS1039 rabbit polyclonal anti-histamine antibody. Fluorescent labeled secondary antibody was used and picture was acquired by confocal imaging.
Histamine antibody - Rabbit polyclonal Ab
Ref: IS1039
Histamine is a widely present natural amino acid known to be involved in different systems including in the control of central nervous and immune functions. The anti-histamine antibody IS1039 is a rabbit polyclonal antibody developped to detect Histamine in various types of samples including whole mounts, cell culture and tissue sections. Importantly, samples must be prepared according to the STAINperfect immunostaining kit A and following the recommended procedure.
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Species reactivity | Reacts with all species |
| Tested samples | whole mounts, cell culture, tissue sections |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µl (approx. 40 tissue sections) |
| Shipping info : | 75.00€ , 4 days |
| Product information | Protocols | References | Technical support | Reviews |
Product overview
| Product name |
Histamine antibody - Rabbit pAb |
| Synonyms |
Anti-Histamine antibody |
| Immunogen | Conjugated Histamine |
| Specificity | When tested in competitive ELISA, the anti-conjugated Histamine antibody did not show any significant cross reactivity with Histidine conjugate. |
| Volume | 50µL |
| Lot number | 160101 |
Storage
| Form |
Liquid |
| Purity | Purified anti-serum |
| Storage |
Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles. |
| Material safety datasheet | Download MSDS |
Protocols
| IF - Cell cultures, Whole mounts, Tissue sections |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments |
Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for histamine staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
References
- Laurino et al. Brain Histamine Modulates the Antidepressant-Like Effect of the 3-Iodothyroacetic Acid (TA1). Front Cell Neurosci. 2019 May 8;13:176. doi: 10.3389/fncel.2019.00176.
- Apolloni A. et al. Histamine Regulates the Inflammatory Profile of SOD1-G93A Microglia and the Histaminergic System Is Dysregulated in Amyotrophic Lateral Sclerosis. Front Immunol. 2017 Nov 30;8:1689. doi: 10.3389/fimmu.2017.01689
- Simons FE et al. Histamine and H1-antihistamines: celebrating a century of progress, J Allergy Clin Immunol. 2011 Dec;128(6):1139-1150.e4. doi: 10.1016/j.jaci.2011.09.005
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