Limited offer: Try 2 ELISA kits for a special price Limited offer: Try 2 ELISA kits for a special price Kynurenine ELISA – typical standard curve Tryptophan ELISA – typical standard curve Example of standard curve obtained with the QA ELISA Kit. In this competitive ELISA, optical density is invertly correlated with Quinolinic Acid levels.

Limited offer: Try 2 ELISA kits for a special price

* Offer limited to one pack per client.

2 Kynurenines ELISA Starter Pack

Ref: ISE-2KYNSP

Test ImmuSmol's ELISA kits against kynurenine pathway metabolites.

The 2 Kynurenines ELISA starter pack enables quantitative measurements of tryptophan catabolism along the kynurenine pathway in large numbers of samples. The two kits included in the pack are ready to use. Highly sensitive, with a minimum sample volume of 25µl, they enable pre-clinical and clinical sample testings.

 

This starter Pack is limited to one per client.


Sample type
Serum, Plasma, Cell supernatant... depending on kit reference
Kit capacity 2 x 96 tests
Sensitivity

LoD KYN: < 47.5 ng/ml

LoD TRP: < 1.2µg/ml
LoD QA:
< 6 ng/ml

Range

KYN: 100 - 10000 ng/ml

TRP: 2.5 - 250 µg/ml
QA: 25 - 2000 ng/ml

Species reactivity Reacts with all species

To make you own starter pack, please select 2 ELISA kits within our range :

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Product information Key steps References Technical support Reviews

Product overview

Product name

2 Kynurenines ELISA Starter Pack

Description Two enzyme immunoassays (ELISA) allowing the quantitative determination of L-Kynurenine, L-Tryptophan or Quinolinic acid in various types of samples.
Format 2 x 96-well plate
Samples Depending on kit reference
Minimum sample volume 20-25 µL
Cross specie-reactivity React with all species
Standard range

KYN: 100 - 10000 ng/ml
TRP: 2.5 - 250 µg/ml

QA: 200 - 2000 µg/ml

Sensitivity

LoD KYN: < 47.5 ng/ml
LoD TRP: < 1.2µg/ml

LoD QA: < 6 ng/ml

Specificity

No significant cross-reactivity was observed with L-Tryptophan, L-Kynurenine and Quinolinic acid analogs. See product pages for L-Kynurenine ELISAL-Tryptophan ELISA and Quinolinic acid ELISA.

Storage Store at 2-8°C for to 6 months.
Datasheets Instructions for use -KYN kitInstructions for use - TRP kitInstructions for use - QA kit
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Key steps

Sample collection & storage

Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands.

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).

Sample preparation

KYN: Sample acylation (90 min)

TRP: Sample precipitation (15 min) and derivatization (2h)
QA: Sample acylation (120 min)

ELISA Antisera overnight incubation, revelation and read steps (1h).
Detailed protocol Instructions for use - KYN kitInstructions for use - TRP kitInstructions for use - QA kit
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References

KYN/TRP ELISA citations :


 

 

 

 

Selected articles on Quinolinic acid:


 

 

 

 

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