Catecholamine ELISA package for research Catecholamine ELISA package for research

Catecholamine ELISA package for research

3 Catecholamines ELISA kit - Any sample - Research

Ref: BA-E-5600

 

This research Catecholamine ELISA kit enables the ultra-sensitive measurement of Epinephrine, Norepinephrine and Dopamine in any biological sample. Intended for research use only, the assay features a lower limit of detection at 6.6 pg/ml and a minimal sample volume as low as 1µl.

 

Sample type
1 - 750 µl, all biological fluids
Samples/kit 40 samples in duplicate
Sensitivity

Epinephrine, Dopamine: 10 pg/ml 
Norepinephrine: 4 pg/ml

Range

Epinephrine, Dopamine: 0 / 0.5 - 80 ng/ml; Norepinephrine: 0 / 0.2 - 32 ng/ml

Assay time Sample extraction, acylation and enzymatic conversion (4 h), ELISA overnight
Species reactivity Reacts with all species
Citations 4
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Product information Key steps References Technical support Reviews

Product overview

Product name

Epinephrine-Norepinephrine ELISA kit - Research

Description Enzyme immunoassay (ELISA) for the ultra-sensitive determination of epinephrine and norepinephrine in biological samples. Research use only.
Format 3 x 96-well plate
Samples Any sample
Sample volume 1 - 750 µl
Cross specie-reactivity React with all species
Standard range Epinephrine, Dopamine: 0 / 0.5 - 80 ng/ml; Norepinephrine: 0 / 0.2 - 32 ng/ml
Sensitivity

Epinephrine, Dopamine: 10 pg/ml 
Norepinephrine: 4 pg/ml

Specificity No significant cross-reactivity was observed with Epinephrine, Norepinephrine and Dopamine analogs such as Metanephrine, Normetanephrine, 3-Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid.
Assay time Sample preparation 4 hours and ELISA overnight
Storage Store at 2-8°C for to 6 months.
Datasheets Instructions for useMaterial safety datasheet
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Key steps

Sample preparation

Sample extraction and acylation (1h45), enzymatic conversion (2h)

ELISA Dopamine, Epinephrine, Norepinephrine antisera overnight incubation, revelation and read steps (1h).
Detailed protocol Download instructions for use
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