Affinity & specificity of 3-Hydroxy-Anthranilic acid antibody
Competitive ELISA demonstrates that low amounts of 3-HAA conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of Anthranilic and Quinolinic acid conjugates do not affect reaction (high specificity).
3-Hydroxyanthranilic acid polyclonal antibodyRef: IS1008
This is the first and only anti-3-Hydroxy-Anthranilic acid (3-HAA) rabbit polyclonal antibody available for research use. Confirmed to be highly specific and affine by competitive ELISA, the antibody is currently being validated for IHC and IF use.
|Valided applications||IHC / IF
|Species reactivity||Reacts with all species|
|References||Not yet cited to our knowledge.
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|Shipping info :||75.00€ , 4 days|
|Product information||Protocols||References||Technical support||Reviews|
|Product name||3-Hydroxyanthranilic acid rabbit polyclonal antibody|
Anti-3-Hydroxy-Anthranilic acid polyclonal antibody
2-Amino-3-hydroxybenzoic acid polyclonal antibody
3-OH-Anthranilic acid polyclonal antibody
3-hydroxanthranilate polyclonal antibody
3-OHAA polyclonal antibody
3-HAA polyclonal antibody
|Immunogen||Conjugated 3-Hydroxyanthranilic acid
|Specificity||When tested by competitive ELISA, the anti- 3-HydroxyAnthranilic acid polyclonal antibody did not show any significant cross reactivity with Anthranilic acid or quinolinic acid conjugates
Reconstitution & storage
|Storage||Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles
|Material safety datasheet
Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections
Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent secondary antibody conjugate.
|Immunohistofluorescence (IHF)||Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent secondary antibody conjugate.|
Optimal working dilutions must be determined by the end-user
|Restrictions||For research use only
Antibody not yet cited. Submit an article and get a 10% discount.
Selected publications on 3-HydroxyAnthranilic acid:
- Lee WS et al. Int Immunopharmacol. The tryptophan metabolite 3-hydroxyanthranilic acid suppresses T cell responses by inhibiting dendritic cell activation. 2013 Nov;17(3):721-6. doi: 10.1016/j.intimp.2013.08.018. Epub 2013 Sep 9.
- Adams S et al. The kynurenine pathway in brain tumor pathogenesis. Cancer Res. 2012 Nov 15;72(22):5649-57. doi: 10.1158/0008-5472.CAN-12-0549. Epub 2012 Nov 9.
- Krause D et al. The tryptophan metabolite 3-hydroxyanthranilic acid plays anti-inflammatory and neuroprotective roles during inflammation: role of hemeoxygenase-1. Am J Pathol. 2011 Sep;179(3):1360-72. doi: 10.1016/j.ajpath.2011.05.048.
- Yan Y et al. IDO upregulates regulatory T cells via tryptophan catabolite and suppresses encephalitogenic T cell responses in experimental autoimmune encephalomyelitis. J Immunol. 2010 Nov 15;185(10):5953-61. doi: 10.4049/jimmunol.1001628. Epub 2010 Oct 13.
- Hayashi T et al. 3-Hydroxyanthranilic acid inhibits PDK1 activation and suppresses experimental asthma by inducing T cell apoptosis. Proc Natl Acad Sci U S A. 2007 Nov 20;104(47):18619-24. Epub 2007 Nov 14.