Affinity & specificity of 3-Hydroxy-Anthranilic acid antibody Affinity & specificity of 3-Hydroxy-Anthranilic acid antibody 3-HydroxyAnthranilic acid (3-HAA)

Affinity & specificity of 3-Hydroxy-Anthranilic acid antibody

Competitive ELISA demonstrates that low amounts of 3-HAA conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of Anthranilic and Quinolinic acid conjugates do not affect reaction (high specificity).

3-Hydroxyanthranilic acid polyclonal antibody

Ref: IS1008

 

This is the first and only anti-3-Hydroxy-Anthranilic acid (3-HAA) rabbit polyclonal antibody available for research use. Confirmed to be highly specific and affine by competitive ELISA, the antibody is currently being validated for IHC and IF use.

Clonality Polyclonal
Host Rabbit
Valided applications IHCIF
Species reactivity Reacts with all species
References Not yet cited to our knowledge.
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Format 50µl
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Product information Protocols References Technical support Reviews

Product overview

 

Product name 3-Hydroxyanthranilic acid rabbit polyclonal antibody
Synonyms

Anti-3-Hydroxy-Anthranilic acid polyclonal antibody

2-Amino-3-hydroxybenzoic acid polyclonal antibody

3-OH-Anthranilic acid polyclonal antibody

3-hydroxanthranilate polyclonal antibody

3-OHAA polyclonal antibody

3-HAA polyclonal antibody

Immunogen Conjugated 3-Hydroxyanthranilic acid
Specificity When tested by competitive ELISA, the anti- 3-HydroxyAnthranilic acid polyclonal antibody did not show any significant cross reactivity with Anthranilic acid or quinolinic acid conjugates
Lot number

140401

 

 

Reconstitution & storage

 

Form

Lyophilized powder

Purity Purified anti-serum
Storage Store at 4°C
Storage buffer

Before use, vial should be resuspended in 50 µL of ultrapure water. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles

Material safety datasheet Download MSDS
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Protocols

Immunocytochemistry(ICC)

Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6)  before initiating IHC staining protocol on paraffin-embedded and frozen sections

Immunohistochemistry (IHC)

Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent secondary antibody conjugate.

Immunohistofluorescence (IHF) Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent secondary antibody conjugate.
Comments

Optimal working dilutions must be determined by the end-user

Restrictions For research use only
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