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3-Methoxytyramine Antibody – Rabbit Polyclonal
Ref: IS1019CatecholaminesPolyclonal AntibodiesICCIFIHC
+Whole mount hindbrain immunostaining of 3MT containing cells.
Anti-3-Metoxytyramine antibody highlights the soma and projections of 3MT positive neurons in the hindbarin of mouse E14.5 embryo. Staining was performed using STAINperfect immunostaining kit A, following the protocol for whole mounts. Secondary antibody (goat anti-rabbit Alexa Fluor® 488) was used and picture was acquired by confocal imaging at high magnification.
DatasheetMSDS
The anti-3-Methoxytyramine (3-MT) antibody IS1019 is a rabbit polyclonal antibody validated for IF in mouse brain tissues (whole mounts, free-floating sections). In samples prepared with the STAINperfect immunostaining kit A, this antibody allows direct 3MT visualization in whole mounts, cell cultures and tissue sections.
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Whole mount, cell culture, tissue sections & ELISA |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µL (approx. 40 tissue sections) |
Product overview
| Product name | 3-Methoxytyramine antibody |
| Synonyms | Anti-3-Methoxytyramine polyclonal antibody 3-MT polyclonal antibody |
| Immunogen | Conjugated 3-Methoxytyramine |
| Specificity | When tested in competitive ELISA, the anti-conjugated 3-Methoxytyramine antibody do not show significant cross-reactivity with several competitors including Tyramine and Dopamine conjugates |
Reconstitution & storage
| Form | Liquid |
| Purity | Purified anti-serum |
| Storage buffer | Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
| IF – Cell cultures, Whole mounts, Tissue sections | Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for 3-MT staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use | Protocol-at-a-glance for cell cultures | Protocol-at-a-glance for whole mounts | Protocol-at-a-glance for tissue sections |
Selected publications on 3-Methoxytyramine:
- van Duinen N et al. Plasma levels of free metanephrines and 3-methoxytyramine indicate a higher number of biochemically active HNPGL than 24-h urinary excretion rates of catecholamines and metabolites. Eur J Endocrinol. 2013 Aug 28;169(3):377-82
- Sotnikova TD et al. The dopamine metabolite 3-methoxytyramine is a neuromodulator. PLoS One. 2010 Oct 18;5(10):e13452.
- Alachkar A, Brotchie JM, Jones OT. Binding of dopamine and 3-methoxytyramine as l-DOPA metabolites to human alpha(2)-adrenergic and dopaminergic receptors. Neurosci Res. 2010 Jul;67(3):245-9.
