D-Glutamate detection in primary mouse cortical neurons
Primary mouse cortical neurons were stained using ImmuSmol anti-D-Glutamate antibody using the STAINperfect immunostaining kit A and following the optimized protocol for cell culture. Alexa fluor 488 conjugated secondary antibodies were used and image was captured by confocal imaging.
D-Glutamate antibody - Rabbit polyclonal AbRef: IS1002
This anti-D-Glutamate rabbit polyclonal antibody IS1002 was developped to ensure superior affinity and specificity. Combined with the STAINperfect immunostaining kit A, this antibody allows direct D-Glutamic acid visualization in cell cultures, whole mounts and tissue sections.
|Species reactivity||Reacts with all species
|Eligible samples||whole mounts, cell culture, tissue sections|
|Staining procedure||STAINperfect immunostaining kit A|
|Format||50µL (approx. 40 tissue sections)|
|Shipping info :||75.00€ , 4 days|
|Product information||Protocols||References||Technical support||Reviews|
D-Glutamate antibody - Rabbit pAb
Anti-D-Glutamic acid antibody
|Specificity||When tested in competitive ELISA, the anti-conjugated D-Glutamate antibody did not show any significant cross reactivity with D-Glutamate analogs, L-Glutamate and D-Aspartate.|
Reconstitution & storage
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.
|Material safety datasheet||Download MSDS|
|IF - Cell cultures, Whole mounts, Tissue sections||
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample
Optimal working dilutions must be determined by the end-user
|Restrictions||For research use only
|Full protocol||Download STAINperfect protocol for D-Glutamate staining|
Instructions for Use
for cell cultures
for whole mounts
for tissue sections
Antibody not yet cited. Submit an article and get a 10% discount.
Selected papers on D-Glutamic acid:
- Dayer MR and Dayer MS. Hypoglycemic Effects of D-glutamic Acid in Diabetic Rats International Journal of Pharmacology, 2010 Nov 6: 930-936.
- Mangas A et al. Immunocytochemical visualization of D-glutamate in the rat brain. Neuroscience. 2007 Jan 19;144(2):654-64. Epub 2006 Nov 3.
- Fisher GH et al. Presence of D-aspartate and D-glutamate in tumor proteins. Cancer Biochem Biophys. 1995 Nov;15(2):79-82.
- Kera Y et al. Presence of free D-glutamate and D-aspartate in rat tissues. Biochim Biophys Acta. 1995 Feb 23;1243(2):283-6.