While amino acids (AA) play essential roles in several metabolic pathways, serving as key building blocks energy sources, the measurement of their free forms in biological fluids figures as a potential biomarker for numerous disease indications (1 – 3). Indeed, significant changes in their levels were highlighted in many pathological contexts, including metabolic deficiencies, liver (4) and renal (5) failures, cancer (6), diabetes (7), muscle dysfunction, and Alzheimer’s disease (8). Therefore, for the quantification of AA free L-enantiomers, we developed a series of easy-to-implement ELISA kits, all of which have been cross-validated with the reference Mass Spectrometry method.
ELISA based quantification of an amino acid array in plasma from healthy donors.
EDTA-plasma samples were collected from three healthy volunteers and then processed for quantification of L-forms of Arginine, Glutamine, Lysine, Phenylalanine, Serine, Tryptophan, and Asparagine using specific ELISA kits.