Urine/Plasma Dopamine ELISA Urine/Plasma Dopamine ELISA Fast Dopamine ELISA: Standard curve

Urine/Plasma Dopamine ELISA

Outside the brain, Dopamine (DA) is mainly secreted by the adrenal medulla. Exerting vasodilator as well as exocrine and paracrine effects, plasma dopamine is then excreted in urine. Elevated levels of urinary dopamine have been found to be associated with urine over-collection, drug effects and neural crest tumours.

Dopamine ELISA kit - Urine-Plasma - Fast Track

Ref: BA-E-6300

 

This Dopamine ELISA kit is tailored for the rapid measurement of Dopamine (DA) in urine and plasma samples. Intended for in vitro diagnostics, this fast-track quantitative immunoassay enables to determine dopamine levels in up to 40 samples within 4 hours.

 

Sample type
10 µl urine, 300 µl plasma
Samples/kit 40 samples in duplicate
Sensitivity

4.5 ng/ml urine, 25 pg/ml plasma

Range 0 / 4.5 - 2 000 ng/ml
Assay time

Sample extraction and acylation (65 min), ELISA (3h)

Species reactivity Reacts with all species
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Product information Key steps References Technical support Reviews

Product overview

Product name

Dopamine ELISA kit - Urine/Plasma - Fast-Track

Description In vitro diagnostics enzyme immunoassay (ELISA) for the rapid determination of Dopamine levels in plasma and urine samples.
Labels IVD, CE
Format 96-well plate
Samples 10 µl urine, 300 µl plasma
Cross specie-reactivity React with all species
Standard range 0 / 4.5 - 2 000 ng/ml
Sensitivity

4.5 ng/ml urine, 25 pg/ml plasma

Specificity

No significant cross-reactivity was observed with Dopamine analogs such as Adrenaline, Noradrenaline, Metanephrine, Normetanephrine, Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid.

Assay time 4 hours
Storage Store at 2-8°C for up to 6 months.
Datasheets Instructions for useMaterial safety datasheet
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Key steps

Sample collection & storage

Plasma: EDTA-Plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 - 8°C; for longer periods (up to 6 months) at - 20°C.

Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C.

Sample preparation

Sample preparation, extractiion and acylation (65min)

ELISA Dopamine antiserum incubation (2H), revelation and read steps (1h).
Detailed protocol Download instructions for use
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