Urine/Plasma Dopamine ELISA
Outside the brain, Dopamine (DA) is mainly secreted by the adrenal medulla. Exerting vasodilator as well as exocrine and paracrine effects, plasma dopamine is then excreted in urine. Elevated levels of urinary dopamine have been found to be associated with urine over-collection, drug effects and neural crest tumours.
Dopamine ELISA kit - Urine-Plasma - Fast Track
Ref: BA-E-6300
This Dopamine ELISA kit is tailored for the rapid measurement of Dopamine (DA) in urine and plasma samples. Intended for in vitro diagnostics, this fast-track quantitative immunoassay enables to determine dopamine levels in up to 40 samples within 4 hours.
| Sample type |
10 µl urine, 300 µl plasma |
| Samples/kit | 40 samples in duplicate |
| Sensitivity |
4.5 ng/ml urine, 25 pg/ml plasma |
| Range | 0 / 4.5 - 2 000 ng/ml |
| Assay time |
Sample extraction and acylation (65 min), ELISA (3h) |
| Species reactivity | Reacts with all species |
| Citations | 1 |
|
Shipping info :
(for 1 kit) | 54.00€ , 5 days |
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name |
Dopamine ELISA kit - Urine/Plasma - Fast-Track |
| Description | In vitro diagnostics enzyme immunoassay (ELISA) for the rapid determination of Dopamine levels in plasma and urine samples. |
| Labels | IVD, CE |
| Format | 96-well plate |
| Samples | 10 µl urine, 300 µl plasma |
| Cross specie-reactivity | React with all species |
| Standard range | 0 / 4.5 - 2 000 ng/ml |
| Sensitivity |
4.5 ng/ml urine, 25 pg/ml plasma |
| Specificity |
No significant cross-reactivity was observed with Dopamine analogs such as Adrenaline, Noradrenaline, Metanephrine, Normetanephrine, Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid. |
| Assay time | 4 hours |
| Storage | Store at 2-8°C for up to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample collection & storage |
Plasma: EDTA-Plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 - 8°C; for longer periods (up to 6 months) at - 20°C. Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C. |
| Sample preparation |
Sample preparation, extractiion and acylation (65min) |
| ELISA | Dopamine antiserum incubation (2H), revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
References
TopTechnical Support Submit a question
No technical question yet. Be the first to ask! TopReviews Submit your review
No reviews yet. Thanks for rating this product. Top
