Epinephrine-Norepinephrine ELISA package
Epinephrine - Norepinephrine ELISA kit - ResearchRef: BA-E-5400
This research Epinephrine/Norepinephrine ELISA kit enables the ultra-sensitive measurement of Adrenaline (Epinephrine) and Noradrenaline (Norepinephrine) in any biological sample. Intended for research use only, the assay features a lower limit of detection at 6.6 pg/ml and a minimal sample volume as low as 1µl.
||1 - 750 µl, all biological fluids
|Samples/kit||40 samples in duplicate|
Epinephrine: 6.6 pg/ml;
Epinephrine: 0 / 0.5 - 80 ng/ml;
|Assay time||Sample extraction, acylation and enzymatic conversion (4 h), ELISA overnight|
|Species reactivity||Reacts with all species|
Shipping info :
(for 1 kit)
|54.00€ , 5 days|
|Product information||Key steps||References||Technical support||Reviews|
Epinephrine-Norepinephrine ELISA kit - Research
|Description||Enzyme immunoassay (ELISA) for the ultra-sensitive determination of epinephrine and norepinephrine in biological samples. Research use only.
|Format||2 x 96-well plate|
|Sample volume||1 - 750 µl
|Cross specie-reactivity||React with all species|
|Standard range||Epinephrine: 0 / 0.5 - 80 ng/ml;
Norepinephrine: 0 / 0.2 - 32 ng/ml
Epinephrine: 6.6 pg/ml;
|Specificity||No significant cross-reactivity was observed with Epinephrine and Norepinephrine analogs such as Dopamine, Metanephrine, Normetanephrine, 3-Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid.|
|Assay time||Sample preparation 4 hours and ELISA overnight|
|Storage||Store at 2-8°C for to 6 months.|
|Datasheets||Instructions for use , Material safety datasheet|
Sample extraction and acylation (1h45), enzymatic conversion (2h)
|ELISA||Epinephrine/Norepinephrine antiserum overnight incubation, revelation and read steps (1h).|
|Detailed protocol||Download instructions for use|
- El Khoury N et al. Upregulation of the hyperpolarization-activated current increases pacemaker activity of the sinoatrial node and heart rate during pregnancy in mice. Circulation. 2013 May 21;127(20):2009-20.
- Jun JC et al. Acute hypoxia induces hypertriglyceridemia by decreasing plasma triglyceride clearance in mice. Am J Physiol Endocrinol Metab. 2012 Aug 1;303(3):E377-88.
- Al-Wadei M. and Schuller HM. Pancreatic cancer cells and normal pancreatic duct epithelial cells express an autocrine catecholamine loop that is activated by nicotinic acetylcholine receptors α3, α5 and α7. Mol Cancer Res. Feb 2012; 10(2): 239–249.