Free Metanephrine – Normetanephrine ELISA kit
Fast I Plasma samples

Ref: BA-E-8300
CatecholaminesDiagnostic ELISA kits (IVD)Research ELISA kits (RUO)ELISA
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ELISA kit for the detection of plasma metanephrines
DatasheetMSDSProtocol

This Free Metanephrines ELISA kit enables the fast measurement of free L-metanephrine and free L-Normetanephrine in plasma samples. Intended for in vitro diagnostics, this quantitative immunoassay is based on a simple and fast sample preparation step, and features a limit of detection below 25pg/ml.

Sample type200µl plasma
Samples/kit80 samples – or 40 samples in duplicate
Sensitivity17pg/ml – Metanephrine
23pg/ml – Normetanephrine
Range36 – 3 600pg/ml – Metanephrine
72 – 7 200pg/ml – Normetanephrine
Assay timeSample preparation and acetylation (30 min), ELISA overnight or 2h
ReactivityReacts with all species
CitationsCited in 4 papers 
Size

2 x 96 wells

Regulatory status

In Vitro Diagnostics (EU), Research Use Only

979.00€

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979.00€

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Product overview

Product nameMetanephrine-Normetanephrine ELISA kit – Plasma – Fast-Track
DescriptionIn vitro diagnostics enzyme immunoassay (ELISA) for the fast determination of free metanephrine (metadrenaline) and free normetanephrine (Normetadrenaline) in plasma samples
LabelsIVD (EU), CE
Format96-well plate
SamplesPlasma
Sample volume200µL
ReactivityReacts with all species
Standard range0 / 36 – 3 600pg/ml – Metanephrine
0 / 72 – 7 200pg/ml – Normetanephrine
Sensitivity

17pg/ml – Metanephrine
23pg/ml – Normetanephrine

SpecificityNo significant cross-reactivity was observed with Metanephrines analogs such as 3-methoxytyramine, Adrenaline, Noradrenaline, Dopamine, VMS, HVMS, L-Dopa, L-Tyrosine, Tyramine and Acetaminophen
Assay timeSample preparation and acetylation 30 min and ELISA overnight
StorageStore at 2-8°C for to 6 months
DatasheetsInstructions for use, Material safety datasheet

 

Sample collection & storageEDTA- or Heparin-plasma sample only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 – 8°C; for longer periods (up to 6 months) at – 20°C
Sample preparationSample precipitation and acetylation (30min)
ELISAMetanephrine/Normetanephrine antisera incubation overnight or for 2h, revelation and read steps (1h)
Detailed protocolDownload instructions for use

Product citations

  • Comparison of plasma metanephrines measured by a commercial immunoassay and urinary catecholamines in the diagnosis of pheochromocytoma
    Check the article
    Authors : Christensen et al., Scandinavian journal of clinical and laboratory investigation
    2011-10
  • Evaluation of an enzyme immunoassay for plasma-free metanephrines in the diagnosis of catecholamine-secreting tumors
    Check the article
    Authors : Procopiou et al., European journal of Endocronology
    2009-04
  • Comparison of free plasma metanephrines enzyme immunoassay with (131)I-MIBG scan in diagnosis of pheochromocytoma
    Check the article
    Authors : Gao et al., Clinical and Experimental Medicine
    2008-07
  • Development of enantioselective immunoassays for free plasma metanephrines
    Check the article
    Authors : Manz et al., Annals of the New York Academy of Sciences
    2004-06

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