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GABA Antibody – Chicken Polyclonal
Ref: IS1036NeuromediatorsPolyclonal AntibodiesICCIFIHC
+GABA and L-Serine in mouse primary neurons culture
Primary mouse cortical neurons were stained using anti-GABA chicken polyclonal antibody (red) and anti-L-Serine polyclonal rabbit antibody (green) using the STAINperfect immunostaining kit A and according to the optimized protocol for cell culture. Co-immunostaining appears in yellow. Alexa fluor 546 and 488 conjugated secondary antibodies respectively were used and picture obtained by confocal imaging.
DatasheetMSDS
The anti-GABA antibody IS1036 is a chicken polyclonal antibody developed for its superior affinity and specificity but also to work in multiplex staining with rabbit polyclonal or mouse monoclonal antibodies. Combined with the STAINperfect immunostaining kit A for samples preparation, this antibody allows visualization of GABA in whole mounts, cell cultures and tissue sections.
| Clonality | Polyclonal |
| Host | Chicken |
| Eligible samples | Whole mounts, cell culture, tissue sections |
| Reactivity | Reacts with all species |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µL (approx. 40 tissue sections) |
| References | Cited in 2 papers |
Product overview
| Product name | GABA antibody – Chicken pAb |
| Synonyms | Anti-gamma-Aminobutyric acid antibody |
| Immunogen | Conjugated GABA |
| Specificity | When tested in competitive ELISA, the anti-conjugated GABA antibody did not show any significant cross reactivity with gamma-Aminobutyric acid analogs, including beta-Alanine and D-Alanine |
| Volume | 50µL |
| Lot number | 150901 |
| Expiration date | 2025-09-01 |
Storage
| Form | Liquid |
| Purity | Purified anti-serum |
| Storage | Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
| IF – Cell cultures, Whole mounts, Tissue sections | Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for GABA staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use | Protocol-at-a-glance for cell cultures | Protocol-at-a-glance for whole mounts | Protocol-at-a-glance for tissue sections |
Product citations
- Gad1 knock-out rats exhibit abundant spike-wave discharges in EEG, exacerbated with valproate treatment
Check the article
Authors : Liu et al., Frontiers in Immunology
2023-09 - Impact of Sleep-Wake-Associated Neuromodulators and Repetitive Low-Frequency Stimulation on Human iPSC-Derived Neurons
Check the article
Authors : Yokoi et al., Frontiers in neuroscience
2019-05
