GABA and Dopamine immunostaining in crayfish eyetalk
Using STAINperfect immunostaining kit A, the anti-GABA (red)chicken polyclonal antibody (IS1036) combined with anti-dopamine (green) rabbit polyclonal antibody (IS1005) demonstrate the presence of GABA and dopamine in crayfish eyetalk. The staining was performed according to the protocol optimized for whole-mount samples. Fluorescent labeled secondary antibodies were used and image obtained by fluorescence imaging.
GABA antibody - Chicken polyclonal AbRef: IS1036
The anti-GABA antibody IS1036 is a chicken polyclonal antibody developed for its superior affinity and specificity but also to work in multiplex staining with rabbit polyclonal or mouse monoclonal antibodies. Combined with the STAINperfect immunostaining kit A for samples preparation, this antibody allows visualization of GABA witin whole mounts, cell culture and tissue sections.
|Eligible samples||whole mounts, cell culture, tissue sections|
|Species reactivity||Reacts with all species|
|Staining procedure||STAINperfect immunostaining kit A
|Format||50µL (approx. 40 tissue sections)|
|Shipping info :||75.00€ , 4 days|
|Product information||Protocols||References||Technical support||Reviews|
GABA antibody - Chicken pAb
|Synonyms||Anti-gamma-Aminobutyric acid antibody
|Specificity||When tested in competitive ELISA, the anti-conjugated GABA antibody did not show any significant cross reactivity with gamma-Aminobutyric acid analogs, including beta-Alanine and D-Alanine.|
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.
|Material safety datasheet||Download MSDS|
|IF - Cell cultures, Whole mounts, Tissue sections||
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample
Optimal working dilutions must be determined by the end-user
|Restrictions||For research use only
|Full protocol||Download STAINperfect protocol for GABA staining|
Instructions for Use
for cell cultures
for whole mounts
for tissue sections