GABA and Dopamine immunostaining in crayfish eyetalk GABA and Dopamine immunostaining in crayfish eyetalk GABA immunostaining in mouse cortical primary neurones GABA and L-Serine in mouse primary neurons culture Affinity & specificity of anti-GABA antibody GABA (γ-Aminobutyric acid)

GABA and Dopamine immunostaining in crayfish eyetalk

Using STAINperfect immunostaining kit A, the anti-GABA (red)chicken polyclonal antibody (IS1036) combined with anti-dopamine (green) rabbit polyclonal antibody (IS1005) demonstrate the presence of GABA and dopamine in crayfish eyetalk. The staining was performed according to the protocol optimized for whole-mount samples. Fluorescent labeled secondary antibodies were used and image obtained by fluorescence imaging.

GABA antibody - Chicken polyclonal Ab

Ref: IS1036

 

The anti-GABA antibody IS1036 is a chicken polyclonal antibody developed for its superior affinity and specificity but also to work in multiplex staining with rabbit polyclonal or mouse monoclonal antibodies. Combined with the STAINperfect immunostaining kit A for samples preparation, this antibody allows visualization of GABA witin whole mounts, cell culture and tissue sections.



Clonality Polyclonal
Host Chicken
Eligible samples whole mounts, cell culture, tissue sections
Species reactivity Reacts with all species
Staining procedure STAINperfect immunostaining kit A
Format 50µL (approx. 40 tissue sections)
440
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Product overview

 


 

Product name

GABA antibody - Chicken pAb

Synonyms Anti-gamma-Aminobutyric acid antibody
Immunogen Conjugated GABA
Specificity When tested in competitive ELISA, the anti-conjugated GABA antibody did not show any significant cross reactivity with gamma-Aminobutyric acid analogs, including beta-Alanine and D-Alanine.
Volume 50µL
Lot number

150901

 

Storage


 

Form

Liquid

Purity Purified anti-serum
Storage

Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.

Material safety datasheet Download MSDS
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Protocols

IF - Cell cultures, Whole mounts, Tissue sections

Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample

Comments

Optimal working dilutions must be determined by the end-user

Restrictions For research use only
Full protocol Download STAINperfect protocol for GABA staining
Protocols-at-a-glance

 

 

 

STAINperfect neurotransmitters instructions for use

STAINperfect neurotransmitter staining protocol cell cultures STAINperfect neurotransmitter staining protocol whole mounts STAINperfect neurotransmitter staining protocol tissue sections
Complete 
Instructions for Use
Protocol-at-a-glance 
for cell cultures
Protocol-at-a-glance 
for whole mounts
Protocol-at-a-glance 
for tissue sections


 

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