GABA and Dopamine immunostaining in crayfish eyetalk
Using STAINperfect immunostaining kit A, the anti-GABA (red)chicken polyclonal antibody (IS1036) combined with anti-dopamine (green) rabbit polyclonal antibody (IS1005) demonstrate the presence of GABA and dopamine in crayfish eyetalk. The staining was performed according to the protocol optimized for whole-mount samples. Fluorescent labeled secondary antibodies were used and image obtained by fluorescence imaging.
GABA antibody - Chicken polyclonal Ab
Ref: IS1036
The anti-GABA antibody IS1036 is a chicken polyclonal antibody developed for its superior affinity and specificity but also to work in multiplex staining with rabbit polyclonal or mouse monoclonal antibodies. Combined with the STAINperfect immunostaining kit A for samples preparation, this antibody allows visualization of GABA witin whole mounts, cell culture and tissue sections.
| Clonality | Polyclonal |
| Host | Chicken |
| Eligible samples | whole mounts, cell culture, tissue sections |
| Species reactivity | Reacts with all species |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µL (approx. 40 tissue sections) |
| Shipping info : | 75.00€ , 4 days |
| Product information | Protocols | References | Technical support | Reviews |
Product overview
| Product name |
GABA antibody - Chicken pAb |
| Synonyms | Anti-gamma-Aminobutyric acid antibody |
| Immunogen | Conjugated GABA |
| Specificity | When tested in competitive ELISA, the anti-conjugated GABA antibody did not show any significant cross reactivity with gamma-Aminobutyric acid analogs, including beta-Alanine and D-Alanine. |
| Volume | 50µL |
| Lot number |
150901 |
Storage
| Form |
Liquid |
| Purity | Purified anti-serum |
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles. |
| Material safety datasheet | Download MSDS |
Protocols
| IF - Cell cultures, Whole mounts, Tissue sections |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments |
Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for GABA staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
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