Cross-validation of Glutamine ELISA and LC/MS data in human plasma samples Cross-validation of Glutamine ELISA and LC/MS data in human plasma samples Typical standard curve of Glutamine ELISA # IS-I-1100 : Glutamine (Gln) ELISA kit

Cross-validation of Glutamine ELISA and LC/MS data in human plasma samples

Gln was quantified in human plasma samples from 40 healthy subjects using IS-I-1100 ELISA kit or by LC/MS. Correlation study showed R2=0.9946, thereby confirming the accuracy of the immunoassay.

Glutamine ELISA kit

Ref: IS-I-1100


Glutamine (Gln) is an abundant non-essential amino acid that is involved in several physiological functions including the regulation of the immune system (1). However, in some disease conditions, Gln metabolism can be highly dysregulated. In cancer, Gln is highly consumed by tumor cells as to fulfill their bioenergetic and biosynthetic requirements for proliferation (2). Targeting Gln catabolism - mainly Glutaminase - is currently investigated as a therapeutic approach to circumvent high Gln consumption by cancer cells and maintain a sufficient level in the microenvironment for an effective anti-tumor immune response (3).

The IS-I-1100 Gln ELISA kit allows for the determination of L-Glutamine in plasma and serum, originating from either preclinical or clinical samples, of a volume as low as 10μL and with a sensitivity of 3µM.


Sample type
Plasma, Serum

96 tests

Sensitivity 3µM
Range 21 - 3125µM
Assay time Sample preparation 3h, ELISA overnight
Species reactivity Reacts with all species
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Product overview

Product name Glutamine ELISA kit
Description Competitive ELISA kit for the quantitative measurement of L-Glutamine (Gln) in plasma and serum samples. For research use only.
Format 96-well plate
Samples Plasma, Serum
Minimal sample volume 10 µL
Species reactivity React with all species
Standard range 21 - 3125 µM



No significant cross-reactivity was observed with Ornithine, Arginine, Asparagine, D-Glutamine and L-Glutamic acid.

Assay time Sample preparation 3h and ELISA overnight
Storage Store at 2-8°C for up to 6 months.
Datasheets Instructions for useMaterial Safety Datasheet


For research use only  - Do not use for diagnostic


Key steps

Sample collection & storage

EDTA Plasma

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).

Sample preparation

Sample preparation (3 hours)

ELISA L-Glutamine antiserum overnight incubation, revelation and read steps (1h).
Detailed protocol Download instructions for use

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