Tumor cell lines from both murine and human origins degrade Glutamine to support their high proliferating rate in a Gls1 dependent manner. Tumor cell lines from both murine and human origins degrade Glutamine to support their high proliferating rate in a Gls1 dependent manner. Murine tumor cell lines degrade Glutamine to support their high proliferating rate in a Gls1 dependent manner. Cross-validation of Glutamine ELISA and LC/MS data in human plasma samples Typical standard curve of Glutamine ELISA # IS-I-1100 : Glutamine (Gln) ELISA kit

Tumor cell lines from both murine and human origins degrade Glutamine to support their high proliferating rate in a Gls1 dependent manner.

Cancer cell lines were seeded and were then left untreated or exposed to a specific Gls1 inhibitor (CB-839 at 1µM). At the end of treatment duration, culture supernatants were collected and processed for Glutamine quantitation by ELISA (#IS-I-1100). As depicted here, the different tumor cell lines harbored a variable Glutamine consumption rate - being higher in murine than in human tumor cell lines. Among the different human cell lines, a higher rate of Glutamine degradation is observed for colorectal (HT29 & HCT116) followed by lung (H1299 & A549) and then breast (BT-549 & MDA-MB231) cancer cell lines. Also, it’s noteworthy that Gls1 blockade by CB-839 only partially reversed Glutamine consumption, thus indicating the participation of another catabolic pathway, more presumably Gls2.

L-Glutamine ELISA kit

Ref: IS-I-1100

 

Glutamine (Gln) is an abundant non-essential amino acid that is involved in several physiological functions including the regulation of the immune system (1). However, in some disease conditions, Gln metabolism can be highly dysregulated. In cancer, Gln is highly consumed by tumor cells as to fulfill their bioenergetic and biosynthetic requirements for proliferation (2). Targeting Gln catabolism - mainly Glutaminase - is currently investigated as a therapeutic approach to circumvent high Gln consumption by cancer cells and maintain a sufficient level in the microenvironment for an effective anti-tumor immune response (3).

The IS-I-1100 Gln ELISA kit allows for the determination of L-Glutamine in plasma and serum, originating from either preclinical or clinical samples, of a volume as low as 10μL and with a sensitivity of 3µM.

 

Sample type
Plasma, Serum, Cell supernatant
Capacity

96 tests

Sensitivity 3µM
Range 21 - 3125µM
Assay time Sample preparation 3h, ELISA overnight
Species reactivity Reacts with all species

 

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Product information Key steps References Technical support Reviews

Product overview

Product name Glutamine ELISA kit
Description Competitive ELISA kit for the quantitative measurement of L-Glutamine (Gln) in plasma and serum samples. For research use only.
Format 96-well plate
Samples Plasma, Serum, Cell supernatant
Minimal sample volume 10 µL
Species reactivity React with all species
Standard range 21 - 3125 µM
Sensitivity

3µM

Specificity

No significant cross-reactivity was observed with Ornithine, Arginine, Asparagine, D-Glutamine and L-Glutamic acid.

Assay time Sample preparation 3h and ELISA overnight
Storage Store at 2-8°C for up to 6 months.
Datasheets Instructions for useMaterial Safety Datasheet

 

For research use only  - Do not use for diagnostic

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Key steps

Sample collection & storage

EDTA Plasma

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).

Sample preparation

Sample preparation (3 hours)

ELISA L-Glutamine antiserum overnight incubation, revelation and read steps (1h).
Detailed protocol Download instructions for use
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