Tumor cell lines from both murine and human origins degrade Glutamine to support their high proliferating rate in a Gls1 dependent manner.
Cancer cell lines were seeded and were then left untreated or exposed to a specific Gls1 inhibitor (CB-839 at 1µM). At the end of treatment duration, culture supernatants were collected and processed for Glutamine quantitation by ELISA (#IS-I-1100). As depicted here, the different tumor cell lines harbored a variable Glutamine consumption rate - being higher in murine than in human tumor cell lines. Among the different human cell lines, a higher rate of Glutamine degradation is observed for colorectal (HT29 & HCT116) followed by lung (H1299 & A549) and then breast (BT-549 & MDA-MB231) cancer cell lines. Also, it’s noteworthy that Gls1 blockade by CB-839 only partially reversed Glutamine consumption, thus indicating the participation of another catabolic pathway, more presumably Gls2.
L-Glutamine ELISA kitRef: IS-I-1100
Glutamine (Gln) is an abundant non-essential amino acid that is involved in several physiological functions including the regulation of the immune system (1). However, in some disease conditions, Gln metabolism can be highly dysregulated. In cancer, Gln is highly consumed by tumor cells as to fulfill their bioenergetic and biosynthetic requirements for proliferation (2). Targeting Gln catabolism - mainly Glutaminase - is currently investigated as a therapeutic approach to circumvent high Gln consumption by cancer cells and maintain a sufficient level in the microenvironment for an effective anti-tumor immune response (3).
The IS-I-1100 Gln ELISA kit allows for the determination of L-Glutamine in plasma and serum, originating from either preclinical or clinical samples, of a volume as low as 10μL and with a sensitivity of 3µM.
||Plasma, Serum, Cell supernatant
|Range||21 - 3125µM|
|Assay time||Sample preparation 3h, ELISA overnight|
|Species reactivity||Reacts with all species|
Special price offer valid until June 30, 2021
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(for 1 kit)
|54.00€ , 5 days|
|Product information||Key steps||References||Technical support||Reviews|
|Product name||Glutamine ELISA kit|
|Description||Competitive ELISA kit for the quantitative measurement of L-Glutamine (Gln) in plasma and serum samples. For research use only.
|Samples||Plasma, Serum, Cell supernatant|
|Minimal sample volume||10 µL
|Species reactivity||React with all species|
|Standard range||21 - 3125 µM|
No significant cross-reactivity was observed with Ornithine, Arginine, Asparagine, D-Glutamine and L-Glutamic acid.
|Assay time||Sample preparation 3h and ELISA overnight|
|Storage||Store at 2-8°C for up to 6 months.|
|Datasheets||Instructions for use, Material Safety Datasheet|
For research use only - Do not use for diagnosticTop
|Sample collection & storage||
Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).
Sample preparation (3 hours)
|ELISA||L-Glutamine antiserum overnight incubation, revelation and read steps (1h).|
|Detailed protocol||Download instructions for use|
Selected articles on Glutamine:
- Michio Miyajima, Amino acids: key sources for immunometabolites and immunotransmitters, Int Immunol, June 2020
- Wang et al. Targeting Glutaminolysis: New Perspectives to Understand Cancer Development and Novel Strategies for Potential Target Therapies, Front Oncol., Oct 2020
- Varghese et al. The glutaminase inhibitor CB-839 (Telaglenastat) enhances the anti-melanoma activity of T cell mediated immunotherapies, Mol Cancer Ther, Dec 2020