Glycine detection in primary mouse cortical neurons Glycine detection in primary mouse cortical neurons Glycine detection in primary mouse cortical neurons - fibers and soma Affinity & specificity of anti-Glycine antibody

Glycine detection in primary mouse cortical neurons

Primary mouse cortical neurons were stained using IS1034 anti-Glycine antibody using the STAINperfect immunostaining kit A and according to the optimized protocol for cell culture. Alexa fluor 488 conjugated secondary antibody was used and picture obtained by confocal imaging.

Glycine antibody - Rabbit polyclonal Ab

Ref: IS1034


The rabbit polyclonal antibody directed against conjugated Glycine - IS1034 - was developped to be highly affine and specific. Combined with the STAINperfect immunostaining kit A, the antibody allows direct Glycine visualization in cell cultures, whole mounts and tissue sections.

 

Clonality Polyclonal antibody
Host Rabbit
Species reactivity Reacts with all species
Tested samples whole mounts, cell culture, tissue sections
Staining procedure STAINperfect immunostaining kit A
Format 50µL (approx. 40 tissue sections)
440
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Product overview

 


 

Product name

Glycine antibody - Rabbit polyclonal Ab

Synonyms

Anti-aminoacetic acid antibody

Immunogen Conjugated Glycine
Specificity When tested in competitive ELISA, the anti-conjugated Glycine antibody did not show any significant cross reactivity with Glycine analogs, including Alanine and Serine conjugates.
Volume 50µL
Lot number

150901

 

Storage

 


 

Form

Liquid

Purity Purified anti-serum
Storage

Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.

Material safety datasheet Download MSDS
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Protocols

 

IF - Cell cultures, Whole mounts, Tissue sections

Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample

Comments

Optimal working dilutions must be determined by the end-user

Restrictions For research use only
Full protocol Download STAINperfect protocol for Glycine staining
Protocols-at-a-glance

 

 

 

STAINperfect neurotransmitters instructions for use

STAINperfect neurotransmitter staining protocol cell cultures STAINperfect neurotransmitter staining protocol whole mounts STAINperfect neurotransmitter staining protocol tissue sections
Complete 
Instructions for Use
Protocol-at-a-glance 
for cell cultures
Protocol-at-a-glance 
for whole mounts
Protocol-at-a-glance 
for tissue sections


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References

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