Glycine detection in primary mouse cortical neurons
Primary mouse cortical neurons were stained using IS1034 anti-Glycine antibody using the STAINperfect immunostaining kit A and according to the optimized protocol for cell culture. Alexa fluor 488 conjugated secondary antibody was used and picture obtained by confocal imaging.
Glycine antibody - Rabbit polyclonal Ab
Ref: IS1034
The rabbit polyclonal antibody directed against conjugated Glycine - IS1034 - was developped to be highly affine and specific. Combined with the STAINperfect immunostaining kit A, the antibody allows direct Glycine visualization in cell cultures, whole mounts and tissue sections.
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Species reactivity | Reacts with all species |
| Tested samples | whole mounts, cell culture, tissue sections |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µL (approx. 40 tissue sections) |
| Shipping info : | 75.00€ , 4 days |
| Product information | Protocols | References | Technical support | Reviews |
Product overview
| Product name |
Glycine antibody - Rabbit polyclonal Ab |
| Synonyms |
Anti-aminoacetic acid antibody |
| Immunogen | Conjugated Glycine |
| Specificity | When tested in competitive ELISA, the anti-conjugated Glycine antibody did not show any significant cross reactivity with Glycine analogs, including Alanine and Serine conjugates. |
| Volume | 50µL |
| Lot number |
150901 |
Storage
| Form |
Liquid |
| Purity | Purified anti-serum |
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles. |
| Material safety datasheet | Download MSDS |
Protocols
| IF - Cell cultures, Whole mounts, Tissue sections |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments |
Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for Glycine staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use |
Protocol-at-a-glance for cell cultures |
Protocol-at-a-glance for whole mounts |
Protocol-at-a-glance for tissue sections |
References
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