Glycine detection in primary mouse cortical neurons
Primary mouse cortical neurons were stained using IS1034 anti-Glycine antibody using the STAINperfect immunostaining kit A and according to the optimized protocol for cell culture. Alexa fluor 488 conjugated secondary antibody was used and picture obtained by confocal imaging.
Glycine antibody - Rabbit polyclonal AbRef: IS1034
The rabbit polyclonal antibody directed against conjugated Glycine - IS1034 - was developped to be highly affine and specific. Combined with the STAINperfect immunostaining kit A, the antibody allows direct Glycine visualization in cell cultures, whole mounts and tissue sections.
|Species reactivity||Reacts with all species
|Tested samples||whole mounts, cell culture, tissue sections|
|Staining procedure||STAINperfect immunostaining kit A|
|Format||50µL (approx. 40 tissue sections)
|Shipping info :||75.00€ , 4 days|
|Product information||Protocols||References||Technical support||Reviews|
Glycine antibody - Rabbit polyclonal Ab
Anti-aminoacetic acid antibody
|Specificity||When tested in competitive ELISA, the anti-conjugated Glycine antibody did not show any significant cross reactivity with Glycine analogs, including Alanine and Serine conjugates.|
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.
|Material safety datasheet||Download MSDS|
|IF - Cell cultures, Whole mounts, Tissue sections||
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample
Optimal working dilutions must be determined by the end-user
|Restrictions||For research use only
|Full protocol||Download STAINperfect protocol for Glycine staining|
Instructions for Use
for cell cultures
for whole mounts
for tissue sections