Research Histamine ELISA
Histamine ELISA kit - Any sample - Research
Ref: BA-E-5800
This research Histamine ELISA kit enables the quantitative measurement of histamine in any biological sample: tissue homogeneates, cell culture supernatants, body fluids, etc. With a minimal sample volume as low as 10µl, this histamine immunoassay allows longitudinal studies in mice and rat models.
| Sample type |
10 µl |
| Samples/kit | 40 samples in duplicate |
| Sensitivity |
0.2 ng/ml |
| Range | 0 / 0.5 - 50 ng/ml |
| Assay time |
Sample acylation (1h30) and ELISA overnight |
| Species reactivity | Reacts with all species |
| Citations | 5 |
|
Shipping info :
(for 1 kit) | 54.00€ , 5 days |
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name |
Histamine ELISA kit - Any sample - Research |
| Description | Enzyme immunoassay (ELISA) for the quantitative determination of histamine levels in biological samples. Research use only. |
| Format | 96-well plate |
| Samples | 10 µl, any sample |
| Cross specie-reactivity | React with all species |
| Standard range | 0 / 0.5 - 50 ng/ml |
| Sensitivity |
0.2 ng/ml |
| Specificity |
No significant cross-reactivity was observed with Histamine analogs such as 3-Methyl-Histamine, Tyramine, L-Phenylalanine, L-Histidine, L-Tyrosine, Tryptamine, 5-Hydroxy-Indole-Acetic Acid, Serotonin |
| Assay time | 1h30 sample acetylation + ELISA overnight |
| Storage | Store at 2-8°C for up to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample preparation |
Sample predilution and acylation (1h30) |
| ELISA | Histamine antiserum incubation (3H or overnight), revelation and read steps (1h30). |
| Detailed protocol | Download instructions for use |
References
Product citation:
- Bertl K et al. Smoking influences salivary histamine levels in periodontal disease. Oral Dis. 2012 May;18(4):410-6.
- Han SC. et al. Fermented fish oil suppresses T helper 1/2 cell response in a mouse model of atopic dermatitis via generation of CD4+CD25+Foxp3+ T cells. BMC Immunology 2012, 13:44
- Yang M, Yang C, Mine Y. Multiple T cell epitope peptides suppress allergic responses in an egg allergy mouse model by the elicitation of forkhead box transcription factor 3- and transforming growth factor-beta-associated mechanisms. Clin Exp Allergy. 2010 Apr;40(4):668-78.
- Husain M, Boermans HJ, Karrow NA. Mesenteric lymph node transcriptome profiles in BALB/c mice sensitized to three common food. BMC Genomics. 2011 Jan 6;12:12.
- Yang M et al. Immunomodulatory effects of egg white enzymatic hydrolysates containing immunodominant epitopes in a BALB/c mouse model of egg allergy. J Agric Food Chem. 2009 Mar 25;57(6):2241-8.
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