Research Histamine ELISA Research Histamine ELISA Histamine ELISA: Standard curve

Research Histamine ELISA

Histamine ELISA kit - Any sample - Research

Ref: BA-E-5800

 

This research Histamine ELISA kit enables the quantitative measurement of histamine in any biological sample: tissue homogeneates, cell culture supernatants, body fluids, etc. With a minimal sample volume as low as 10µl, this histamine immunoassay allows longitudinal studies in mice and rat models.

Sample type
10 µl
Samples/kit 40 samples in duplicate
Sensitivity

0.2 ng/ml

Range 0 / 0.5 - 50 ng/ml
Assay time

Sample acylation (1h30) and ELISA overnight

Species reactivity Reacts with all species
Citations 5
440
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Product information Key steps References Technical support Reviews

Product overview

Product name

Histamine ELISA kit - Any sample - Research

Description Enzyme immunoassay (ELISA) for the quantitative determination of histamine levels in biological samples. Research use only.
Format 96-well plate
Samples 10 µl, any sample
Cross specie-reactivity React with all species
Standard range 0 / 0.5 - 50 ng/ml
Sensitivity

0.2 ng/ml

Specificity

No significant cross-reactivity was observed with Histamine analogs such as 3-Methyl-Histamine, Tyramine, L-Phenylalanine, L-Histidine, L-Tyrosine, Tryptamine, 5-Hydroxy-Indole-Acetic Acid, Serotonin

Assay time 1h30 sample acetylation + ELISA overnight
Storage Store at 2-8°C for up to 6 months.
Datasheets Instructions for useMaterial safety datasheet
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Key steps

Sample preparation

Sample predilution and acylation (1h30)

ELISA Histamine antiserum incubation (3H or overnight), revelation and read steps (1h30).
Detailed protocol Download instructions for use
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