Cross-validation of Homoarginine ELISA and LC/MS data in human plasma samples
hArg was quantified in human plasma samples from 40 healthy subjects either using IS-I-0500 ELISA kit or by liquid chromatography–mass spectrometry (LC/MS). Correlation coefficient of 0.964 confirms the accuracy of the immunoassay.
HomoArginine ELISA kitRef: IS-I-0500
HomoArginine (hArg) is a basic non-essential amino acid that exerts beneficial effects on vascular homeostasis, more likely through the increase of nitric oxide (NO) that can be explained through two mechanisms since hArg i) serves as a precursor of NO and ii) favors the elevation of L-Arg - main substrate of NO synthase - by inhibiting the enzyme Arginase.
The homoArginine (hArg) ELISA kit allows for the determination of hArg in serum and plasma samples working with a sample volume of 20μL. The kit is easy to implement and well suited for both preclinical and clinical studies.
|Range||0.32 - 12.5µM|
|Assay time||Sample preparation 3h, ELISA overnight|
|Species reactivity||Reacts with all species|
Shipping info :
(for 1 kit)
|54.00€ , 5 days|
|Product information||Key steps||References||Technical support||Reviews|
|Product name||HomoArginine ELISA kit|
|Description||Competitive ELISA kit for the quantitative measurement of Homoarginine (hARG) in serum and plasma samples. For research use only.
|Minimal sample volume||20 µL
|Species reactivity||React with all species|
|Standard range||0.32 - 12.5 µM|
No significant cross-reactivity was observed with L-Arginine, Agmatine, L-Citruline, L-Ornithine, ADMA and SDMA.
|Assay time||Sample preparation 3h and ELISA overnight|
|Storage||Store at 2-8°C for up to 6 months.|
|Datasheets||Instructions for use, Material Safety Datasheet|
|Sample collection & storage||
Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands.
Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).
Sample preparation (3 hours)
|ELISA||Homoarginine antiserum overnight incubation, revelation and read steps (1h).|
|Detailed protocol||Download instructions for use|
Selected articles on Homoarginine:
- Karetnikova et al. Is Homoarginine a Protective Cardiovascular Risk Factor?Arterioscler Thromb Vasc Biol. 2019
- Wetzel et al. L-Homoarginine supplementation prevents diabetic kidney damage.Physiol Rep. 2019
- Helm et al. Plasma Homoarginine Concentrations According to Use of Hormonal Contraception.Sci Rep. 2018
- Atzler et al. L-homoarginine and cardiovascular disease. Curr Opin Clin Nutr Metab Care. 2015