Cross-validation of Kynurenic Acid ELISA and LC/MS data in human serum samples
Human serum samples (n=40) were processed for Kynurenic Acid measurement by mean of both KYNA ELISA kit and liquid chromatography–mass spectrometry (LC/MS). As depicted, results obtained with ELISA and LC/MS are highly correlated (R Squared=0,9902).
Kynurenic acid ELISA kit
Ref: IS-I-0200
Kynurenic acid (KYNA) is a downstream metabolite produced along the Kynurenine Pathway well-known to possess several neuromodulatory functions, as well as immuno-modulatory properties. Measurement of KYNA in biological samples has been presented as a possible biomarker in several diseases including neurological or psychiatric disorders.
The Kynurenic Acid ELISA kit is optimized for the quantitation of Kynurenic Acid (KYNA) within serum samples. This easy-to-use competitive assay is well-suited for both small and large series of samples. Working with a 50µL sample volume, this assay enables pre-clinical and clinical sample testings.
| Sample type |
Serum |
| Capacity | 96 tests |
| Sensitivity |
0.53 ng/ml |
| Range | 1.40 - 74 ng/ml |
| Assay time | Sample derivatization 90 min, ELISA overnight |
| Species reactivity | Reacts with all species |
|
Shipping info :
(for 1 kit) | 54.00€ , 5 days |
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name |
Kynurenic Acid ELISA kit |
| Description | Competitive ELISA kit for the quantitative measurement of Kynurenic Acid in serum samples. For research use only. |
| Format | 96-well plate |
| Samples | Serum |
| Minimal sample volume | 50 µL |
| Species reactivity | React with all species |
| Standard range | 1.40 - 74 ng/mL |
| Sensitivity |
0.53 ng/mL |
| Specificity |
No significant cross-reactivity was observed with Kynurenic Acid analogs such as Quinolinic Acid, Xanthurenic acid, Kynurenine, Picolinic acid, 3Hydroxy-Anthranilic Acid |
| Assay time | Sample derivatization 90 min and ELISA overnight |
| Storage | Store at 2-8°C for up to 6 months. |
| Datasheets | Instructions for use, Material Safety Datasheet |
Key steps
| Sample collection & storage |
Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). |
| Sample preparation |
Sample derivatization (90 min) |
| ELISA | Kynurenic acid antiserum overnight incubation, revelation and read steps (1h). |
| Detailed protocol | Download instructions for use |
References
Selected articles on Kynurenic Acid:
- Pedraz-Petrozzi et al. Effects of inflammation on the kynurenine pathway in schizophrenia - a systematic review. J Neuroinflammation. 2020
- Achtyes et al. Inflammation and kynurenine pathway dysregulation in post-partum women with severe and suicidal depression. Brain Behav Immun. 2020
- Walczak et al. Kynurenic acid and cancer: facts and controversies. Cell Mol Life Sci. 2019
- Lim et al. Kynurenine pathway metabolomics predicts and provides mechanistic insight into multiple sclerosis progression. Sci Rep. 2017
- Plitman et al. Kynurenic Acid in Schizophrenia: A Systematic Review and Meta-analysis. Schizophr Bull. 2017
- Wirthgen et al. Kynurenic Acid: The Janus-Faced Role of an Immunomodulatory Tryptophan Metabolite and Its Link to Pathological Conditions. Front Immunol. 2017
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