Cross-validation of Kynurenic Acid ELISA and LC/MS data in human serum samples Cross-validation of Kynurenic Acid ELISA and LC/MS data in human serum samples Typical standard curve of Kynurenic acid ELISA Kynurenic Acid ELISA kit IS-I-0200 Kynurenic acid (KYNA) is a metabolite produced along the Kynurenine Pathway

Cross-validation of Kynurenic Acid ELISA and LC/MS data in human serum samples

Human serum samples (n=40) were processed for Kynurenic Acid measurement by mean of both KYNA ELISA kit and liquid chromatography–mass spectrometry (LC/MS). As depicted, results obtained with ELISA and LC/MS are highly correlated (R Squared=0,9902).

Kynurenic acid ELISA kit

Ref: IS-I-0200

Kynurenic acid (KYNA) is a downstream metabolite produced along the Kynurenine Pathway well-known to possess several neuromodulatory functions, as well as immuno-modulatory properties. Measurement of KYNA in biological samples has been presented as a possible biomarker in several diseases including neurological or psychiatric disorders.

The Kynurenic Acid ELISA kit is optimized for the quantitation of Kynurenic Acid (KYNA) within serum samples. This easy-to-use competitive assay is well-suited for both small and large series of samples. Working with a 50µL sample volume, this assay enables pre-clinical and clinical sample testings.

 

Sample type
Serum
Capacity 96 tests
Sensitivity

0.53 ng/ml

Range 1.40 - 74 ng/ml
Assay time Sample derivatization 90 min, ELISA overnight
Species reactivity Reacts with all species
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Product overview

Product name

Kynurenic Acid ELISA kit

Description Competitive ELISA kit for the quantitative measurement of Kynurenic Acid in serum samples. For research use only.
Format 96-well plate
Samples Serum
Minimal sample volume 50 µL
Species reactivity React with all species
Standard range 1.40 - 74 ng/mL
Sensitivity

0.53 ng/mL

Specificity

No significant cross-reactivity was observed with Kynurenic Acid analogs such as Quinolinic Acid, Xanthurenic acid, Kynurenine, Picolinic acid, 3Hydroxy-Anthranilic Acid

Assay time Sample derivatization 90 min and ELISA overnight
Storage Store at 2-8°C for up to 6 months.
Datasheets Instructions for useMaterial Safety Datasheet
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Key steps

Sample collection & storage

Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands.

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).

Sample preparation

Sample derivatization (90 min)

ELISA Kynurenic acid antiserum overnight incubation, revelation and read steps (1h).
Detailed protocol Download instructions for use
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