Kynurenine/Kynurenic Acid/Quinolinic Acid ELISA pack (3-KYNs ELISA pack)
The three kits included in the pack are ready to use. Highly sensitive, with a minimum sample volume of 25-50µL, they enable pre-clinical and clinical sample testings.
Kynurenine/Kynurenic Acid/Quinolinic Acid ELISA pack
(3-KYNs ELISA pack)
Ref: ISE-220102
The Kynurenine/Kynurenic Acid/Quinolinic acid ELISA pack enables quantitative measurements of tryptophan catabolism along the kynurenine pathway in large numbers of samples. The three kits included in the pack are ready to use. Highly sensitive, with a minimum sample volume of 25-50µL, they enable pre-clinical and clinical sample testings.
| Sample type |
Plasma, Serum |
| Kit capacity | 3 x 96 tests |
| Sensitivity |
LoD KYN: 47.5 ng/ml LoD KYNA: 0.53 ng/ml LoD QA: 6 ng/ml |
| Range |
KYN: 100 - 10000 ng/ml KYNA: 1.40 - 74 ng/ml |
| Species reactivity | Reacts with all species |
|
Shipping info :
(for 1 kit) | 85.00€ , 5 days |
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name |
Kynurenine/Kynurenic Acid/Quinolinic Acid ELISA pack |
| Description | Three enzyme immunoassays (ELISA) allowing the quantitative determination of L-Kynurenine, Kynurenic acid and Quinolinic acid in serum samples. |
| Labels | CE |
| Format | 3 x 96-well plate |
| Samples | Serum |
| Minimum sample volume | 20-50 µL |
| Cross specie-reactivity | React with all species |
| Standard range |
KYN: 100 - 10000 ng/ml QA: 200 - 2000 µg/ml |
| Sensitivity |
LoD KYN: 47.5 ng/ml LoD QA: 6 ng/ml |
| Specificity |
No significant cross-reactivity was observed analogs for each kit. See product pages for L-Kynurenine ELISA, Kynurenic acid ELISA and Quinolinic acid ELISA. |
| Storage | Store at 2-8°C for to 6 months. |
| Datasheets | Instructions for use - KYN kit, Instructions for use - KYNA kit, Instructions for use - QA kit |
Key steps
| Sample collection & storage |
Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months). |
| Sample preparation |
KYN: Sample acylation (90 min) KYNA: Sample derivatization (90 min) |
| ELISA | Antisera overnight incubation, revelation and read steps (1h). |
| Detailed protocol | Instructions for use - KYN kit, Instructions for use - KYNA kit, Instructions for use - QA kit |
References
KYN ELISA citations :
Selected articles on Kynurenic acid:
- Pedraz-Petrozzi et al. Effects of inflammation on the kynurenine pathway in schizophrenia - a systematic review. J Neuroinflammation. 2020
- Achtyes et al. Inflammation and kynurenine pathway dysregulation in post-partum women with severe and suicidal depression. Brain Behav Immun. 2020
- Walczak et al. Kynurenic acid and cancer: facts and controversies. Cell Mol Life Sci. 2019
- Lim et al. Kynurenine pathway metabolomics predicts and provides mechanistic insight into multiple sclerosis progression. Sci Rep. 2017
- Plitman et al. Kynurenic Acid in Schizophrenia: A Systematic Review and Meta-analysis. Schizophr Bull. 2017
- Wirthgen et al. Kynurenic Acid: The Janus-Faced Role of an Immunomodulatory Tryptophan Metabolite and Its Link to Pathological Conditions. Front Immunol. 2017
Selected articles on Quinolinic acid:
- Sundaram et al. Quinolinic acid toxicity on oligodendroglial cells: relevance for multiple sclerosis and therapeutic strategies. J Neuroinflammation. 2014 Dec 13
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