Kynurenine pathway metabolites – Antibody Starter Pack for IHC

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Product information	Protocols	References	Technical support	Reviews

Product overview

Product name	Kynurenine pathway metabolites - antibody starter pack for IHC/IF
Immunogen	Conjugated amino acid metabolites
Clonality	Mouse monoclonal or rabbit polyclonal antibodies
Specificity	Each antibody was tested for affinity and specificity by competitive ELISA. ELISA data are available on product pages.
Specie reactivity	React with all species
Format	3 x 25µl

Reconstitution & storage

Form	Lyophilized powder
Storage	Store at 4°C
Storage buffer	Before use, vial should be resuspended in 25 µL of ultrapure water. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.

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Protocols

Immunohistochemistry (IHC)	Dilute at 1:200-1:2000. Perform heat antigen retrieval before initiating IHC staining protocol on paraffin-embedded and frozen sections.
Immunofluorescence (IF)	Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent dye-conjugated secondary antibody.
Comments	Optimal working dilutions must be determined by the end-user.
Restrictions	For research use only.

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References

Antibodies are not yet cited. Submit an article and get a 10% discount.

Selected publications on kynurenine pathway metabolites:

Hoshi M, Matsumoto K, Ito H, Ohtaki H, Arioka Y, Osawa Y, Yamamoto Y, Matsunami H, Hara A, Seishima M, Saito K. L-tryptophan-kynurenine pathway metabolites regulate type I IFNs of acute viral myocarditis in mice. J Immunol. 2012 Apr 15;188(8):3980-7. doi: 10.4049/jimmunol.1100997. Epub 2012 Mar 14.

Berthon C, Fontenay M, Corm S, Briche I, Allorge D, Hennart B, Lhermitte M, Quesnel B. Metabolites of tryptophan catabolism are elevated in sera of patients with myelodysplastic syndromes and inhibit hematopoietic progenitor amplification. Leuk Res. 2013 May;37(5):573-9. doi: 10.1016/j.leukres.2013.02.001. Epub 2013 Feb 28.

Schwarcz R, Bruno JP, Muchowski PJ, Wu HQ. Kynurenines in the mammalian brain: when physiology meets pathology. Nat Rev Neurosci. 2012 Jul;13(7):465-77. doi: 10.1038/nrn3257.

Pilotte L, Larrieu P, Stroobant V, Colau D, Dolusic E, Frédérick R, De Plaen E, Uyttenhove C, Wouters J, Masereel B, Van den Eynde BJ. Reversal of tumoral immune resistance by inhibition of tryptophan 2,3-dioxygenase. Proc Natl Acad Sci U S A. 2012 Feb 14;109(7):2497-502. doi: 10.1073/pnas.1113873109. Epub 2012 Jan 30.

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Technical Support Submit a question

Asked
2014-04-02

What was your negative control for these small metabolite specific antibodies in immunohistological staining? Lei Huang

Answered
2014-04-02

Many thanks for your question Lei. We use non-relevant mouse igG as negative control for each staining.

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