Kynurenine/Tryptophan/Quinolinic acid ELISA pack Kynurenine/Tryptophan/Quinolinic acid ELISA pack Kynurenine - Tryptophan - Quinolinic Acid ELISA standard curves

Kynurenine/Tryptophan/Quinolinic acid ELISA pack

Kynurenine/Tryptophan/Quinolinic Acid ELISA pack

Ref: ISE-222701

The Kynurenine/Tryptophan/Quinolinic acid ELISA pack enables quantitative measurements of tryptophan catabolism along the kynurenine pathway in large numbers of samples. The three kits included in the pack are ready to use. Highly sensitive, with a minimum sample volume of 25µl, they enable pre-clinical and clinical sample testings.


Sample type
Plasma, Serum
Kit capacity 3 x 96 tests
Sensitivity

LoD KYN: < 47.5 ng/ml

LoD TRP: < 1.2µg/ml

LoD QA: < 6 ng/ml

Range

KYN: 100 - 10000 ng/ml

TRP: 2.5 - 250 µg/ml
QA: 25 - 2000 ng/ml

Species reactivity Reacts with all species
1300
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Product information Key steps References Technical support Reviews

Product overview

Product name

Kynurenine/Tryptophan/Quinolinic acid ELISA pack

Description Three enzyme immunoassays (ELISA) allowing the quantitative determination of L-Kynurenine, L-Tryptophan and Quinolinic acid in serum samples.
Labels CE
Format 3 x 96-well plate
Samples Serum
Minimum sample volume 20-25 µL
Cross specie-reactivity React with all species
Standard range

KYN: 100 - 10000 ng/ml
TRP: 2.5 - 250 µg/ml

QA: 200 - 2000 µg/ml

Sensitivity

LoD KYN: < 47.5 ng/ml
LoD TRP: < 1.2µg/ml

LoD QA: < 6 ng/ml

Specificity

No significant cross-reactivity was observed with L-Tryptophan, L-Kynurenine and Quinolinic acid analogs. See product pages for L-Kynurenine ELISAL-Tryptophan ELISA and Quinolinic acid ELISA.

Storage Store at 2-8°C for to 6 months.
Datasheets Instructions for use -KYN kitInstructions for use - TRP kit, Instructions for use - QA kit
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Key steps

Sample collection & storage

Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).

Sample preparation

KYN: Sample acylation (90 min)

TRP: Sample precipitation (15 min) and derivatization (2h)
QA: Sample acylation (120 min)

ELISA Antisera overnight incubation, revelation and read steps (1h).
Detailed protocol Instructions for use - KYN kitInstructions for use - TRP kitInstructions for use - QA kit
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References

KYN/TRP ELISA citations :


 

 

 

 

Selected articles on Quinolinic acid:


 

 

 

 

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