Measurement of plasma levels of both KYNA and QA in mice treated with KMO inhibitors.

Kynurenic acid/Quinolinic acid ELISA pack

KYNA and QA along the Kynurenine Pathway

Measurement of plasma levels of both KYNA and QA in mice treated with KMO inhibitors.

Mice were treated with two selective KMO inhibitors – Ro-618048 & UPF648 – at two different doses each. Plasma samples were collected before treatment and at 1h30 and 6h post-treatment. KYNA and QA levels were then quantified by mean of ELISA (IS-I-0200 and IS-I-0100). As depicted above, blockade of KMO time- and dose-dependently favors the degradation of Kynurenine into KYNA. Interestingly, no major effect is observed on QA level thus suggesting compensatory mechanism to maintain stable circulating QA level.

Kynureninic Acid/Quinolinic Acid ELISA pack (KYNA/QA ELISA pack)

Ref: ISE-0102

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Through the degradation of L-Tryptophan, the Kynurenine pathway (KP) generates a series of catabolites collectively known as Kynurenines, which can exert immunomodulatory and/or neuroactive properties. Known to respectively harbor neuroprotective and neurotoxic functions, Kynurenic (KYNA) and Quinolinic (QA) acids are found to be dysregulated in several neuroinflammatory disorders including Parkinson and Alzheimer disease but also Amyotrophic Lateral Sclerosis and Multiple Sclerosis.

The two kits included in the pack are ready to use, optimized for the quantitation of Kynurenic Acid (KYNA) and Quinolinic Acid (QA) within serum and plasma samples. These easy-to-use competitive assays are well-suited for both small and large series of samples. Working with a 25-50µL sample volume, these assays enable pre-clinical and clinical sample testings.

Sample type	Serum, Plasma
Kit capacity	2 x 96 tests
Sensitivity	LoD KYNA: 0.53 ng/ml LoD QA: 6 ng/ml
Range	KYNA: 1.40 - 74 ng/ml QA: 25 - 2000 ng/ml
Species reactivity	Reacts with all species

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Product overview

Product name	Kynurenic Acid/Quinolinic Acid ELISA pack
Description	Two enzyme immunoassays (ELISA) allowing the quantitative determination of Kynurenic acid and Quinolinic acid in serum samples.
Labels	CE
Format	2 x 96-well plate
Samples	Serum, Plasma
Minimum sample volume	25-50 µL
Cross specie-reactivity	React with all species
Standard range	KYNA: 1.40 - 74 ng/ml QA: 25 - 2000 ng/ml
Sensitivity	LoD KYNA: 0.53 ng/ml LoD QA: 6 ng/ml
Specificity	No significant cross-reactivity was observed analogs for each kit. See product pages for Kynurenic acid ELISAand Quinolinic acid ELISA.
Storage	Store at 2-8°C for to 6 months.
Datasheets	Instructions for use - KYNA kit, Instructions for use - QA kit Safety datasheet - KYNA kit, Safety datasheet QA kit

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Key steps

Sample collection & storage	Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).
Sample preparation	KYNA: Sample derivatization (90 min) QA: Sample acylation (120 min)
ELISA	Antisera overnight incubation, revelation and read steps (1h).
Detailed protocol	Instructions for use - KYNA kit, Instructions for use - QA kit