Measurement of plasma levels of both KYNA and QA in mice treated with KMO inhibitors. Measurement of plasma levels of both KYNA and QA in mice treated with KMO inhibitors. KYNA / QA ELISA standard curves Kynurenic acid/Quinolinic acid ELISA pack KYNA and QA along the Kynurenine Pathway

Measurement of plasma levels of both KYNA and QA in mice treated with KMO inhibitors.

Mice were treated with two selective KMO inhibitors – Ro-618048 & UPF648 – at two different doses each. Plasma samples were collected before treatment and at 1h30 and 6h post-treatment. KYNA and QA levels were then quantified by mean of ELISA (IS-I-0200 and IS-I-0100). As depicted above, blockade of KMO time- and dose-dependently favors the degradation of Kynurenine into KYNA. Interestingly, no major effect is observed on QA level thus suggesting compensatory mechanism to maintain stable circulating QA level.

Kynureninic Acid/Quinolinic Acid ELISA pack (KYNA/QA ELISA pack)

Ref: ISE-0102

Through the degradation of L-Tryptophan, the Kynurenine pathway (KP) generates a series of catabolites collectively known as Kynurenines, which can exert immunomodulatory and/or neuroactive properties. Known to respectively harbor neuroprotective and neurotoxic functions, Kynurenic (KYNA) and Quinolinic (QA) acids are found to be dysregulated in several neuroinflammatory disorders including Parkinson and Alzheimer disease but also Amyotrophic Lateral Sclerosis and Multiple Sclerosis.

 

The two kits included in the pack are ready to use, optimized for the quantitation of Kynurenic Acid (KYNA) and Quinolinic Acid (QA) within serum and plasma samples. These easy-to-use competitive assays are well-suited for both small and large series of samples. Working with a 25-50µL sample volume, these assays enable pre-clinical and clinical sample testings.


Sample type
Serum, Plasma
Kit capacity 2 x 96 tests
Sensitivity

LoD KYNA: 0.53 ng/ml

LoD QA: 6 ng/ml

Range

KYNA: 1.40 - 74 ng/ml

QA: 25 - 2000 ng/ml

Species reactivity Reacts with all species
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Product information Key steps References Technical support Reviews

Product overview

Product name

Kynurenic Acid/Quinolinic Acid ELISA pack

Description Two enzyme immunoassays (ELISA) allowing the quantitative determination of Kynurenic acid and Quinolinic acid in serum samples.
Labels CE
Format 2 x 96-well plate
Samples Serum, Plasma
Minimum sample volume 25-50 µL
Cross specie-reactivity React with all species
Standard range

KYNA: 1.40 - 74 ng/ml

QA: 25 - 2000 ng/ml

Sensitivity

LoD KYNA: 0.53 ng/ml

LoD QA: 6 ng/ml

Specificity

No significant cross-reactivity was observed analogs for each kit. See product pages for Kynurenic acid ELISAand Quinolinic acid ELISA.

Storage Store at 2-8°C for to 6 months.
Datasheets

Instructions for use - KYNA kitInstructions for use - QA kit

Safety datasheet - KYNA kit, Safety datasheet QA kit

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Key steps

Sample collection & storage

Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months).

Sample preparation

KYNA: Sample derivatization (90 min)

QA: Sample acylation (120 min)

ELISA Antisera overnight incubation, revelation and read steps (1h).
Detailed protocol Instructions for use - KYNA kitInstructions for use - QA kit
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References

Selected articles on Kynurenic acid:


Selected articles on Quinolinic acid:

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