Affinity & specificity of anti- L-Glutamine antibody Affinity & specificity of anti- L-Glutamine antibody L-Glutamine

Affinity & specificity of anti- L-Glutamine antibody

Competitive ELISA demonstrates that low amounts of L-Glutamine conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of L-Ornithine, L-Arginine and L-Asparagine (L-Glutamine competitors) do not affect reaction (high specificity).

L-Glutamine antibody

Ref: IS1060


Validated by direct and competitive ELISA, this rabbit polyclonal antibody recognizes L-Glutamine at 1/10 000 working dilution, while displaying no significant cross-reactivity with competitors such as L-Ornithine, L-Arginine and L-Asparagine.

In combination with optimal samples preparation using STAINperfect immunostaining kit A, this antibody could allow direct L-Glutamine visualization in various type of samples (cell culture, tissue...).

Clonality Polyclonal
Host Rabbit
Species reactivity Reacts with all species
Validated applications
ELISA
Staining procedure STAINperfect immunostaining kit A
References Not yet cited to our knowledge.
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Product information Protocols References Technical support Reviews

Product overview

 

Product name

L-Glutamine - Rabbit pAb

Synonyms

Anti-L-Glutamine antibody

Anti-Levoglutamide antibody

Anti-L-(+)-Glutamine antibody

Anti-Glutamic acid amide antibody

Anti-(S)-2,5-Diamino-5-oxopentanoic acid antibody

Immunogen Conjugated L-Ornithine
Specificity When tested in competitive ELISA, the anti-conjugated L-Ornithine antibody did not show any significant cross reactivity with L-Ornithine competitors: L-Ornithine, L-Arginine and L-Asparagine.
Lot number

180801

 

Storage

 

Form

Liquid

Purity Purified anti-serum
Storage Store at 4°C
Storage buffer

Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.

Material safety datasheet Download MSDS
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Protocols

IF - Cell cultures, Whole mounts, Tissue sections


Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample

Comments

Optimal working dilutions must be determined by the end-user

Restrictions For research use only
Full protocol Download STAINperfect protocol
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References

Antibody not yet cited. Submit an article and get a 10% discount.

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