Affinity & specificity of anti-L-Homoarginine antibody Affinity & specificity of anti-L-Homoarginine antibody L-Homoarginine

Affinity & specificity of anti-L-Homoarginine antibody

Competitive ELISA demonstrates that low amounts of L-Homoarginine conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of Asymetric DiMethyl Arginine (ADMA), Symetric DiMethyl Arginine (SDMA) and L-Arginine (L-Homoarginine competitors) do not affect reaction (high specificity).

L-Homoarginine antibody

Ref: IS1025


Validated by direct and competitive ELISA, this rabbit polyclonal antibody recognizes L-Homoarginine at 1/10 000 working dilution, while displaying no significant cross-reactivity with competitors such as Asymetric DiMethyl Arginine (ADMA), Symetric DiMethyl Arginine (SDMA) and L-Arginine.

In combination with optimal sample preparation using STAINperfect immunostaining kit A, this antibody could allow direct L.Homoarginine visualization in various type of samples (cell culture, tissue...).


Clonality Polyclonal
Host Rabbit
Species reactivity Reacts with all species
Validated applications
ELISA
Staining procedure STAINperfect immunostaining kit A
References Not yet cited to our knowledge.
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Format 50µl
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Product overview

 

Product name

L-Homoarginine - Rabbit pAb

Synonyms

Anti-L-Homoarginine antibody

Anti-(S)-2-Amino-6-guanidinohexanoic acid hydrochloride antibody

Immunogen Conjugated L-Homoarginine
Specificity When tested in competitive ELISA, the anti-conjugated L-Homoarginine antibody did not show any significant cross reactivity with L-Homoarginine competitors, Asymetric DiMethyl Arginine (ADMA), Symetric DiMethyl Arginine (SDMA) and L-Arginine.
Lot number

150901

 

Storage

 

Form

Liquid

Purity Purified anti-serum
Storage Store at 4°C
Storage buffer

Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.

Material safety datasheet Download MSDS
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Protocols

IF - Cell cultures, Whole mounts, Tissue sections


Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample

Comments

Optimal working dilutions must be determined by the end-user

Restrictions For research use only
Full protocol Download STAINperfect protocol

 

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References

Antibody not yet cited. Submit an article and get a 10% discount.

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