Affinity & specificity of anti-L-Homoarginine antibody
Competitive ELISA demonstrates that low amounts of L-Homoarginine conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of Asymetric DiMethyl Arginine (ADMA), Symetric DiMethyl Arginine (SDMA) and L-Arginine (L-Homoarginine competitors) do not affect reaction (high specificity).
L-Homoarginine antibodyRef: IS1025
Validated by direct and competitive ELISA, this rabbit polyclonal antibody recognizes L-Homoarginine at 1/10 000 working dilution, while displaying no significant cross-reactivity with competitors such as Asymetric DiMethyl Arginine (ADMA), Symetric DiMethyl Arginine (SDMA) and L-Arginine.
In combination with optimal sample preparation using STAINperfect immunostaining kit A, this antibody could allow direct L.Homoarginine visualization in various type of samples (cell culture, tissue...).
|Species reactivity||Reacts with all species|
|Staining procedure||STAINperfect immunostaining kit A|
|References||Not yet cited to our knowledge.
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|Product information||Protocols||References||Technical support||Reviews|
L-Homoarginine - Rabbit pAb
Anti-(S)-2-Amino-6-guanidinohexanoic acid hydrochloride antibody
|Specificity||When tested in competitive ELISA, the anti-conjugated L-Homoarginine antibody did not show any significant cross reactivity with L-Homoarginine competitors, Asymetric DiMethyl Arginine (ADMA), Symetric DiMethyl Arginine (SDMA) and L-Arginine.|
|Storage||Store at 4°C|
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles.
|Material safety datasheet||Download MSDS|
IF - Cell cultures, Whole mounts, Tissue sections
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample
Optimal working dilutions must be determined by the end-user
|Restrictions||For research use only
|Full protocol||Download STAINperfect protocol|