Affinity & specificity of anti-L-Homoarginine antibody
Competitive ELISA demonstrates that low amounts of L-Homoarginine conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of Asymetric DiMethyl Arginine (ADMA), Symetric DiMethyl Arginine (SDMA) and L-Arginine (L-Homoarginine competitors) do not affect reaction (high specificity).
L-Homoarginine antibody
Ref: IS1025
Validated by direct and competitive ELISA, this rabbit polyclonal antibody recognizes L-Homoarginine at 1/10 000 working dilution, while displaying no significant cross-reactivity with competitors such as Asymetric DiMethyl Arginine (ADMA), Symetric DiMethyl Arginine (SDMA) and L-Arginine.
In combination with optimal sample preparation using STAINperfect immunostaining kit A, this antibody could allow direct L.Homoarginine visualization in various type of samples (cell culture, tissue...).
| Clonality | Polyclonal |
| Host | Rabbit |
| Species reactivity | Reacts with all species |
| Validated applications |
ELISA |
| Staining procedure | STAINperfect immunostaining kit A |
| References | Not yet cited to our knowledge. Submit content and get a 10% discount! |
| Format | 50µl |
| Shipping info : | 75.00€ , 4 days |
| Product information | Protocols | References | Technical support | Reviews |
Product overview
| Product name |
L-Homoarginine - Rabbit pAb |
| Synonyms |
Anti-L-Homoarginine antibody Anti-(S)-2-Amino-6-guanidinohexanoic acid hydrochloride antibody |
| Immunogen | Conjugated L-Homoarginine |
| Specificity | When tested in competitive ELISA, the anti-conjugated L-Homoarginine antibody did not show any significant cross reactivity with L-Homoarginine competitors, Asymetric DiMethyl Arginine (ADMA), Symetric DiMethyl Arginine (SDMA) and L-Arginine. |
| Lot number |
150901 |
Storage
| Form |
Liquid |
| Purity | Purified anti-serum |
| Storage | Store at 4°C |
| Storage buffer |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles. |
| Material safety datasheet | Download MSDS |
Protocols
IF - Cell cultures, Whole mounts, Tissue sections
|
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
|
| Comments |
Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol |
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