L-Kynurenine visualization in human intestinal immune cells by IF
Immunofluorescence staining reveals L-Kynurenine accumulation in specific immune cells in human colon tissue. Paraffin-embedded tissue section was subjected to pH=6 antigen retrieval followed by overnight incubation with primary antibody (dilution 1/250). After incubation with Alexa-488 conjugated secondary Ab, epifluorescence microscopy (100X) was used to visualize IF staining.
L-Kynurenine antibody
Ref: IS003
Monoclonal anti-L-Kynurenine antibody 3D4-F2 enabled to illustrate, for the first time, the presence of L-kynurenine in human brain and tumor samples by IHC and IF. Recently, our anti-kynurenine antibody was used to stratify cancer patients for kynurenine pathway activity (open-access PLOS ONE article).
| Clonality | Monoclonal antibody (clone 3D4-F2) |
| Host | Mouse |
| Validated applications | IHC / IF |
| Specie reactivity | Reacts with all species |
| References | 5 citations |
| Format | 50 µL |
| Shipping info : | 9440.25¥ , 4 days |
| Product information | Protocols | References | Technical support | Reviews |
Product overview
| Product name | L-Kynurenine antibody |
| Synonyms |
(S)-Kynurenine antibody L-2-Amino-4-(2-aminophenyl)-4-oxobutanoic acid antibody Kynurenin antibody 3-Anthraniloyl-L-alanine antibody, |
| Immunogen | Conjugated L-Kynurenine |
| Isotype | IgG1 k chain |
| Clone | clone 3D4-F2 |
| Specificity | When tested in competitive ELISA, the anti-L-Kynurenine antibody did not show any significant cross reactivity with L-Tryptophan, 3-hydroxy-DL-Kynurenine, Kynurenic acid, Anthranilic acid or 3-hydroxyAnthranilic acid conjugates |
| Lot number |
140201 |
Storage
| Form |
Liquid |
| Purity | Purified IgG |
| Concentration |
0,5 mg/ml |
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles. |
| Material safety datasheet | Download MSDS |
Protocols
| Immunohistochemistry (IHC) |
Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections |
| Immunofluorescence (IF) |
Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Before staining, perform heat antigen retrieval |
| Comments |
Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
References
- Product citation: Yamamoto et al. Kynurenine signaling through the aryl hydrocarbon receptor maintains the undifferentiated state of human embryonic stem cells. Sci Signal. 2019 Jun 25
- Product citation: Takenaka et al. Control of tumor-associated macrophages and T cells in glioblastoma via AHR and CD39. Nature Neuroscience 2019 April 8
- Product citation:Maliniemi et al. Biological and clinical significance of tryptophan-catabolizing enzymes in cutaneous T-cell lymphomas. Oncoimmunology. 2017 Feb 10.
- Product citation: Puccetti et al. Accumulation of an endogenous tryptophan-derived metabolite in colorectal and breast cancers. PLoS One. 2015 Apr 16;10(4)
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