Plasma Normetanephrine ELISA
Normetanephrine ELISA kit - Plasma - Fast-trackRef: BA-E-8200
This Normetanephrine ELISA kit enables the fast measurement of free L-Normetanephrine in plasma samples. Intended for in vitro diagnostics, this quantitative immunoassay is based on a simple and fast sample preparation protocol, and offers a limit of detection as low as 23 pg/ml.
||500 µl plasma
|Samples/kit||40 samples in duplicate|
|Range||0 / 48 - 4 800 pg/ml|
|Assay time||Sample preparation and acetylation (30 min), ELISA overnight|
|Species reactivity||Reacts with all species|
Shipping info :
(for 1 kit)
|62.66$ , 5 days|
|Product information||Key steps||References||Technical support||Reviews|
Normetanephrine ELISA kit - Plasma - Fast-Track
|Description||In vitro diagnostics enzyme immunoassay (ELISA) for the fast determination of free normetanephrine (normetadrenaline) in plasma samples.
|Sample volume||500 µL
|Cross specie-reactivity||React with all species|
|Standard range||0 / 48 - 4 800 pg/ml|
|Specificity||No significant cross-reactivity was observed with Normetanephrine analogs such as Metanephrine, 3-methoxytyramine, Adrenaline, Noradrenaline, Dopamine, VMS, HVMS, L-Dopa, L-Tyrosine, Tyramine and Acetaminophen.|
|Assay time||Sample preparation and acetylation 30 min and ELISA overnight|
|Storage||Store at 2-8°C for to 6 months.|
|Datasheets||Instructions for use , Material safety datasheet|
|Sample collection & storage||
EDTA- or citrate-plasma sample only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 - 8°C; for longer periods (up to 6 months) at - 20°C.
Sample precipitation and acetylation (30min)
|ELISA||Normetanephrine antiserum overnight incubation, revelation and read steps (1h).|
|Detailed protocol||Download instructions for use|
- Manz B et al. Development of enantioselective immunoassays for free plasma metanephrines. Ann N Y Acad Sci. 2004 Jun;1018:582-7.
- Gao YC et al. Comparison of free plasma metanephrines enzyme immunoassay with (131)I-MIBG scan in diagnosis of pheochromocytoma. Clin Exp Med. 2008 Jun;8(2):87-91.
- Procopiou M et al. Evaluation of an enzyme immunoassay for plasma-free metanephrines in the diagnosis of catecholamine-secreting tumors. Eur J Endocrinol. 2009 Jul;161(1):131-40.
- Christensen TT et al. Comparison of plasma metanephrines measured by a commercial immunoassay and urinary catecholamines in the diagnosis of pheochromocytoma. Scand J Clin Lab Invest. 2011 Dec;71(8):695-700.