Affinity & specificity of Picolinic acid polyclonal antibody
Competitive ELISA demonstrates that low amounts of Picolinic acid conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of Quinolinic acid conjugate do not affect reaction (high specificity).
Picolinic acid antibodyRef: IS1015
This is the first and only anti-Picolinic acid antibody available for research use. Confirmed to be highly specific and affine by competitive ELISA, this rabbit polyclonal antibody is undergoing validation for IHC and IF use.
|Applications||IHC / IF|
|Species reactivity||Reacts with all species|
|References||Not yet cited to our knowledge.
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|Shipping info :||75.00€ , 4 days|
|Product information||Protocols||References||Technical support||Reviews|
|Product name||Picolinic acid antibody|
pyridine-2-carboxylic acid antibody
|Immunogen||Conjugated picolinic acid
|Specificity||When tested in competitive ELISA, the anti-Picolinic acid antibody did not show any significant cross reactivity with analog Quinolinic acid.|
|Storage||Store at 4°C|
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles
|Material safety datasheet||Download MSDS|
Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections
Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent secondary antibody conjugate.
|Immunohistofluorescence (IHF)||Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent secondary antibody conjugate.|
Optimal working dilutions must be determined by the end-user
|Restrictions||For research use only
Selected publications about Picolinic acid:
- Zavitsanou K et al. Effect of maternal immune activation on the kynurenine pathway in preadolescent rat offspring and on MK801-induced hyperlocomotion in adulthood: Amelioration by COX-2 inhibition. Brain Behav Immun. 2014 May 27. pii: S0889-1591(14)00133-0. doi: 10.1016/j.bbi.2014.05.011.
- Reyes Ocampo J et al. Kynurenines with neuroactive and redox properties: relevance to aging and brain diseases. Oxid Med Cell Longev. 2014;2014:646909. doi: 10.1155/2014/646909. Epub 2014 Feb 17.
- Zuwala-Jagiello, J et al. Picolinic Acid in Patients with Chronic Hepatitis C Infection: A Preliminary Report. Mediators Inflamm. 2012;2012:762863. doi: 10.1155/2012/762863. Epub 2012 Apr 10.