Affinity & specificity of Picolinic acid polyclonal antibody Affinity & specificity of Picolinic acid polyclonal antibody Picolinic acid

Affinity & specificity of Picolinic acid polyclonal antibody

Competitive ELISA demonstrates that low amounts of Picolinic acid conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of Quinolinic acid conjugate do not affect reaction (high specificity).

Picolinic acid antibody

Ref: IS1015

 

This is the first and only anti-Picolinic acid antibody available for research use. Confirmed to be highly specific and affine by competitive ELISA, this rabbit polyclonal antibody is undergoing validation for IHC and IF use.

 

Clonality Polyclonal
Host Rabbit
Applications IHC / IF
Species reactivity Reacts with all species
References Not yet cited to our knowledge.
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Product information Protocols References Technical support Reviews

Product overview

 

Product name Picolinic acid antibody
Synonyms

pyridine-2-carboxylic acid antibody

Immunogen Conjugated picolinic acid
Specificity When tested in competitive ELISA, the anti-Picolinic acid antibody did not show any significant cross reactivity with analog Quinolinic acid.
Lot number

140801

 

Storage

 

Form

Liquid

Purity Purified anti-serum
Storage Store at 4°C
Storage buffer

Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles

Material safety datasheet Download MSDS
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Protocols

Immunocytochemistry(ICC)

Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6)  before initiating IHC staining protocol on paraffin-embedded and frozen sections

Immunohistochemistry (IHC)

Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent secondary antibody conjugate.

Immunohistofluorescence (IHF) Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent secondary antibody conjugate.
Comments

Optimal working dilutions must be determined by the end-user

Restrictions For research use only
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