Rapid Fish Histamine test - HistaSure™
Ref: FC-L-3200
This Histamine dipstick assay kit is tailored for the rapid screening of fish samples (fresh, canned, salted, ...) exceeding the regulatory 50ppm histamine defect action level. Performed within 10 minutes, the assay does not require any special equipment, and allows reusing samples for further analysis by ELISA.
(Only available for customers outside of France)
| Sample type |
10g fish |
| Number of tests | 24 dipsticks |
| Sensitivity/Range | Cut-off at at 50 ppm (easily adjustable) |
| Assay time |
12 min |
| Species reactivity | Reacts with all species |
|
Shipping info :
(for 1 kit) | 54.00€ , 5 days |
| Product information | Key steps | References | Technical support | Reviews |
Product overview
| Product name |
Histasure™ rapid dipstick test for fish samples |
| Description | Dipstick assay (immunogold) for rapid screening of fish samples for >50ppm histamine levels (regulatory histamine defect action level). |
| Format | 24 lateral flow dipstick |
| Samples | 10g fish sample (fresh, canned, salted, in oil, ...) |
| Cross specie-reactivity | React with all species |
| Standard range | Cut-off at 50 ppm - Adjustable |
| Assay time | 12 min |
| Storage | Store at 2-8°C for up to 6 months. |
| Datasheets | Instructions for use , Material safety datasheet |
Key steps
| Sample preparation |
Weigh 10 g of fish sample. Add 240 ml distilled water and homogenize for 1-2 minutes in a grinder or blender. Filter or centrifuge the homogenate. |
| Sample acylation |
Pipet 100 µl of the filtered homogenate into the acylation buffer tubes. Screw down the acylation buffer tubes with the acylation caps and mix vigorously by hand. Incubate the tubes for 5 minutes at RT. |
| Assay | Pipet 100 µl of acylated samples into the running buffer tubes. Cap the tubes and mix gently. Pipet 100 µl from the running buffer tubes to the microtiter wells, add the lateral flow device (blue part) and incubate for 5 min at room temperature. Read result within 5 minutes. |
| Detailed protocol | Download instructions for use |
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