Rapid Fish Histamine test - HistaSure™

Ref: FC-L-3200

 

This Histamine dipstick assay kit is tailored for the rapid screening of fish samples (fresh, canned, salted, ...) exceeding the regulatory 50ppm histamine defect action level. Performed within 10 minutes, the assay does not require any special equipment, and allows reusing samples for further analysis by ELISA.

(Only available for customers outside of France)

Sample type
10g fish
Number of tests 24 dipsticks
Sensitivity/Range Cut-off at at 50 ppm (easily adjustable)
Assay time

12 min

Species reactivity Reacts with all species
£149.37
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(for 1 kit)
44.81£ , 5 days
Product information Key steps References Technical support Reviews

Product overview

Product name

Histasure™ rapid dipstick test for fish samples

Description Dipstick assay (immunogold) for rapid screening of fish samples for >50ppm histamine levels (regulatory histamine defect action level).
Format 24 lateral flow dipstick
Samples 10g fish sample (fresh, canned, salted, in oil, ...)
Cross specie-reactivity React with all species
Standard range Cut-off at 50 ppm - Adjustable
Assay time 12 min
Storage Store at 2-8°C for up to 6 months.
Datasheets Instructions for useMaterial safety datasheet
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Key steps

Sample preparation

Weigh 10 g of fish sample. Add 240 ml distilled water and homogenize for 1-2 minutes in a grinder or blender. Filter or centrifuge the homogenate.

Sample acylation

Pipet 100 µl of the filtered homogenate into the acylation buffer tubes. Screw down the acylation buffer tubes with the acylation caps and mix vigorously by hand. Incubate the tubes for 5 minutes at RT.

Assay Pipet 100 µl of acylated samples into the running buffer tubes. Cap the tubes and mix gently. Pipet 100 µl from the running buffer tubes to the microtiter wells, add the lateral flow device (blue part) and incubate for 5 min at room temperature. Read result within 5 minutes.
Detailed protocol Download instructions for use
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