Fast Serotonin (5-HT) ELISA Fast Serotonin (5-HT) ELISA Fast 5-HT ELISA: Standard curve

Fast Serotonin (5-HT) ELISA

Serotonin (5-hydroxytryptamine) is a monoamine neurotransmitter derived from tryptophan metabolism. Produced by enterochromaffin cells in the gut and serotonergic neurons in the brain, serotonin is stored and transported by platelets in the blood. It is then excreted in urine directly, or in the form of 5-HIAA, its main metabolite. Altered serotonin levels in serum, urine and platelets are associated with carcinoid tumors (high concentrations) and CNS disorders such as autism, depression and suicidal schizophrenia (low concentrations).

Serotonin ELISA kit - Serum, Urine, Platelets - Fast Track

Ref: BA-E-8900

 

This Serotonin (5-HT) ELISA kit is tailored for the rapid measurement of 5-hydroxytryptamine in serum, urine and platelets. Intended for in vitro diagnostics, this fast-track quantitative immunoassay enables the determination of serotonin levels in up to 40 samples within 75 minutes.

 

Sample type
25 µl serum, urine, platelets
Samples/kit 40 samples in duplicate
Sensitivity

5 ng/ml

Range 0 / 15 - 2 500 ng/ml
Assay time

Sample acylation (15 min), ELISA (1h)

Species reactivity Reacts with all species
Citations 3
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Product information Key steps References Technical support Reviews

Product overview

Product name

Serotonin ELISA kit - Serum, Urine, Platelets - Fast Track

Description In vitro diagnostics enzyme immunoassay (ELISA) for the fast determination of 5-hydroxytryptamine (5-HT, Serotonin) in serum, urine or platelets.
Labels IVD, CE
Format 96-well plate
Samples 25 µl serum, urine, platelets
Cross-species reactivity React with all species
Standard range 0 / 15 - 2 500 ng/ml
Sensitivity

5 ng/ml

Specificity

No significant cross-reactivity was observed with Serotonin analogs such as Tryptamine, Melatonin, 5-Hydroxyindole acetic acid, Phenylalanine, Histidine, Tyramine and 5-Hydroxytryptophan.

Assay time 1h15
Storage Store at 2-8°C for up to 6 months.
Datasheets Instructions for useMaterial safety datasheet
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Key steps

Sample collection & storage

Serum: Do not use haemolytic and especially lipemic samples. Storage: up to 6 hours at 2 - 8°C; for longer periods (up to 6 months) at - 20°C.

Plasma: EDTA-Plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 - 8°C; for longer periods (up to 6 months) at - 20°C.

Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C.

Sample preparation

Sample acylation (15min)

ELISA Serotonin antiserum incubation (30min), revelation and read steps (30min).
Detailed protocol Download instructions for use
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