Antibodies & immunoassays for small molecules 3 Catecholamines ELISA kit – Research
Plasma & Urine samples
Ref: BA-E-6600R
CatecholaminesResearch ELISA kits (RUO)ELISA
+Plasma/Urine catecholamines ELISA kit: Dopamine, Adrenaline, Noradrenaline
DatasheetMSDSProtocol
This Catecholamine ELISA kit is tailored for the rapid measurement of Adrenaline (Epinephrine), Noradrenaline (Norepinephrine) and Dopamine in urine and plasma samples. Intended for research use only, this fast-track quantitative immunoassay enables to determine catecholamine levels in up to 40 samples within 4 hours.
| Sample type | 10µl urine, 300µl plasma |
| Samples/kit | 40 samples in duplicate |
| Sensitivity | Epinephrine: 0.3ng/ml urine, 10pg/ml plasma Norepinephrine: 1.5ng/ml urine, 40pg/ml plasma Dopamine: 4.5ng/ml urine, 25pg/ml plasma |
| Range | Epinephrine: 1 – 200ng/ml Norepinephrine: 5 – 1 000ng/ml Dopamine: 4.5 – 2 000ng/ml |
| Assay time | Sample extraction and acylation (65 min), ELISA (3h) |
| Reactivity | Reacts with all species |
| Citations | 2 |
Product overview
| Product name | 3 catecholamines ELISA kit – Urine/Plasma – Fast-Track |
| Description | Competitive ELISA kit for the quantitative measurement of Adrenaline, Noradrenaline and Dopamine levels in plasma and urine samples. For research use only |
| Labels | RUO |
| Format | 3×96-well plate |
| Samples | Urine, Plasma |
| Sample volume | 10µl urine, 300µl plasma |
| Reactivity | Reacts with all species |
| Standard range | Epinephrine: 0 / 1 – 200ng/ml Norepinephrine: 0 / 5 – 1 000ng/ml Dopamine: 0 / 4.5 – 2 000ng/ml |
| Sensitivity |
Epinephrine: 0.3ng/ml urine, 10pg/ml plasma Norepinephrine: 1.5ng/ml urine, 40pg/ml plasma Dopamine: 4.5ng/ml urine, 25pg/ml plasma |
| Specificity | No significant cross-reactivity was observed with Dopamine, Epinephrine and Norepinephrine analogs such as Dopamine, Metanephrine, Normetanephrine, 3-Methoxytyramine, 3-Methoxy-4-hydroxyphenylglycol, Tyramine, Phenylalanine, Caffeinic acid, L-Dopa, Homovanillic acid, Tyrosine and 3-Methoxy-4-hydroxymandelic acid |
| Assay time | 4 hours |
| Storage | Store at 2-8°C for to 6 months |
| Datasheets | Instructions for use, Material safety datasheet |
| The kit for in-vitro-diagnostic (IVD) use can be ordered with the catalog no. BA E-6600. |
| Sample collection & storage | Plasma: EDTA-Plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 – 8°C; for longer periods (up to 6 months) at – 20°C
Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15mL of 6M HCl. Storage: for longer periods (up to 6 months) at -20°C |
| Sample preparation | Sample preparation, extraction and acylation (65min) |
| ELISA | Dopamine, Epinephrine, Norepinephrine antisera incubation (2H), revelation and read steps (1h) |
| Detailed protocol | Download instructions for use |
Product citation
- Antidepressant- and anxiolytic-like activities of Rosmarinus officinalis extract in rodent models: Involvement of oxytocinergic system
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Authors : Sasaki et al., Biomedicine & Pharmacotherapy
2021-10 - Effects of Sodium-Glucose Cotransporter 2 Inhibition on Glucose Metabolism, Liver Function, Ascites, and Hemodynamics in a Mouse Model of Nonalcoholic Steatohepatitis and Type 2 Diabetes
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Authors : Koichi et al., Journal of Diabetes Research
2020-12 - Resuscitation From Hemorrhagic Shock With Fresh and Stored Blood and Polymerized Hemoglobin
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Authors : Williams et al., Shock
2020-10 - Balance between oxygen transport and blood rheology during resuscitation 2 from hemorrhagic shock with polymerized bovine hemoglobin
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Authors : Williams et al., Journal of apllied physiology
2020-07 - Myogenic vasoconstriction requires G 12/G 13 and LARG to maintain local and systemic vascular resistance
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Authors : Chennupati et al., eLife
2019-09 - Increased arterial pressure in mice with overexpression of the ADHD candidate gene calcyon in forebrain
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Authors : Elmarakby et al., PloS one
2019-02 - Hyperfiltration in ubiquitin C-terminal hydrolase L1-deleted mice
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Authors : Boisvert et al., Clinical Science
2018-07 - Emotional states of love moderate the association between catecholamines and female sexual responses in the laboratory
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Authors : Dundon et al., Journal of sexual medicine
2012-05 - Renal kallikrein excretion and epigenetics in human acute kidney injury: expression, mechanisms and consequences
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Authors : Kang et al., BMC Nephrology
2011-06
