Antibodies & immunoassays for small molecules Free Metanephrine – Normetanephrine ELISA kit – Research
Plasma samples
Ref: BA-E-8300R
CatecholaminesResearch ELISA kits (RUO)ELISA
+ELISA kit for the detection of plasma metanephrines
DatasheetMSDSProtocol
This Free Metanephrines ELISA kit enables the fast measurement of free L-metanephrine and free L-Normetanephrine in plasma samples. Intended for research use only, this quantitative immunoassay is based on a simple and fast sample preparation step, and features a limit of detection below 25pg/ml.
| Sample type | 200µl plasma |
| Samples/kit | 80 samples – or 40 samples in duplicate |
| Sensitivity | Metanephrine : 17pg/ml Normetanephrine : 23pg/ml |
| Range | Metanephrine : 36 – 3 600pg/ml Normetanephrine : 72 – 7 200pg/ml |
| Assay time | Sample preparation and acetylation (30 min), ELISA overnight or 2h |
| Reactivity | Reacts with all species |
| Citations | 4 |
Product overview
| Product name | Metanephrine-Normetanephrine ELISA kit – Plasma – Fast-Track |
| Description | Competitive ELISA kit for the quantitative measurement of free metanephrine (metadrenaline) and free normetanephrine (normetadrenaline) levels in plasma samples. For research use only |
| Labels | RUO |
| Format | 96-well plate |
| Samples | Plasma |
| Sample volume | 200µL |
| Reactivity | Reacts with all species |
| Standard range | 0 / 36 – 3 600pg/ml – Metanephrine 0 / 72 – 7 200pg/ml – Normetanephrine |
| Sensitivity |
17pg/ml – Metanephrine |
| Specificity | No significant cross-reactivity was observed with Metanephrines analogs such as 3-methoxytyramine, Adrenaline, Noradrenaline, Dopamine, VMS, HVMS, L-Dopa, L-Tyrosine, Tyramine and Acetaminophen |
| Assay time | Sample preparation and acetylation 30 min and ELISA overnight |
| Storage | Store at 2-8°C for to 6 months |
| Datasheets | Instructions for use, Material safety datasheet |
| The kit for in-vitro-diagnostic (IVD) use can be ordered with the catalog no. BA E-8300. |
| Sample collection & storage | EDTA- or Heparin-plasma sample only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 – 8°C; for longer periods (up to 6 months) at – 20°C |
| Sample preparation | Sample precipitation and acetylation (30min) |
| ELISA | Metanephrine/Normetanephrine antisera incubation overnight or for 2h, revelation and read steps (1h) |
| Detailed protocol | Download instructions for use |
Product citation
- Comparison of plasma metanephrines measured by a commercial immunoassay and urinary catecholamines in the diagnosis of pheochromocytoma
Check the article
Authors : Christensen et al., Scandinavian journal of clinical and laboratory investigation
2011-10 - Evaluation of an enzyme immunoassay for plasma-free metanephrines in the diagnosis of catecholamine-secreting tumors
Check the article
Authors : Procopiou et al., European journal of Endocronology
2009-04 - Comparison of free plasma metanephrines enzyme immunoassay with (131)I-MIBG scan in diagnosis of pheochromocytoma
Check the article
Authors : Gao et al., Clinical and Experimental Medicine
2008-07 - Development of enantioselective immunoassays for free plasma metanephrines
Check the article
Authors : Manz et al., Annals of the New York Academy of Sciences
2004-06
