Antibodies & immunoassays for small molecules Histamine ELISA kit – Research
Plasma & Urine samples
Ref: BA-E-1000R
NeuromediatorsResearch ELISA kits (RUO)ELISA
+Plasma/Urine Histamine ELISA
DatasheetMSDSProtocol
This Histamine ELISA kit is tailored for the quantitative measurement of histamine in urine and plasma samples. Intended for research use only, the immunoassay can be combined with the add-on kit BA-E-1100, to quantify histamine release in whole blood.
| Sample type | 10µl urine, 25µl plasma |
| Samples/kit | 40 samples in duplicate |
| Sensitivity | 0.3ng/ml urine, 0.12ng/ml plasma |
| Range | 0.5 – 50ng/ml |
| Assay time | Sample acylation (1h) and ELISA overnight |
| Reactivity | Reacts with all species |
| Citations | 4 |
Product overview
| Product name | Histamine ELISA kit – Urine and Plasma samples |
| Description | Competitive ELISA kit for the quantitative measurement of Histamine levels in urine and plasma samples. For research use only |
| Labels | RUO |
| Format | 96-well plate |
| Samples | 10µl urine, 25µl plasma |
| Reactivity | Reacts with all species |
| Standard range | 0 / 0.5 – 50ng/ml |
| Sensitivity |
0.12ng/ml plasma, 0.3ng/ml urine |
| Specificity | No significant cross-reactivity was observed with Histamine analogs such as 3-Methyl-Histamine, Tyramine, L-Phenylalanine, L-Histidine, L-Tyrosine, Tryptamine, 5-Hydroxy-Indole-Acetic Acid, Serotonin |
| Assay time | 1h sample acetylation + ELISA overnight |
| Storage | Store at 2-8°C for up to 6 months |
| Datasheets | Instructions for use, Material safety datasheet |
| The kit for in-vitro-diagnostic (IVD) use can be ordered with the catalog no. BA E-1000. |
| Sample collection & storage | Plasma: EDTA or Heparin plasma samples only. Do not use haemolytic or lipemic samples. Storage: up to 6 hours at 2 – 8°C; for longer periods (up to 6 months) at – 20°C
Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C |
| Sample preparation | Sample acylation (1h) |
| ELISA | Histamine antiserum incubation (3H or overnight), revelation and read steps (1h) |
| Detailed protocol | Download instructions for use |
Product citation
- Increased serum diamine oxidase activity in nonallergic patients with migraine
Check the article
Authors : Garcia-Martin et al, European journal of Clinical Investigation
2022-02 - Inactivated antithombin as anticoagulant reversal in a rat model of cardiopulmonary bypass: a potent and potentially safer alternative to protamine
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Authors : Bianchini et al., Britis journal of Haematology
2018-01 - TCTP/HRF pathway and angiogenesis: a feasible intercourse in chronic lymphocytic leukemia
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Authors : Yagcı et al., Leukemia Research
2013-02 - The effect of the extract of Crocus sativus and its constituent safranal, on lung pathology and lung inflammation of ovalbumin sensitized guinea-pigs
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Authors : Boskabady et al., Photomedicine
2012-06 - H2-receptor-mediated vasodilation contributes to postexercise hypotension
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Authors : McCord et al., Journal of Applied Physiology
2005-09
