Antibodies & immunoassays for small molecules L-Kynurenine ELISA kit – Research
Ref: BA-E-2200R
ELISAKynurenine pathwayTryptophan metabolismResearch ELISA kits (RUO)
+Kynurenine ELISA – typical standard curve Determination of L-Kynurenine levels in cell cultures through microdialysis collection and by ELISA quantification Kynurenine ELISA cross-validation by GC/MS in human plasma samples L-Kynurenine quantification by ELISA in 20µl mice plasma samples Determination of L-Kynurenine levels in CT26 cell cultures by ELISA Kynurenine ELISA – typical standard curve Kynurenine ELISA – typical standard curve
Example of standard curve obtained with the Kynurenine ELISA Kit. In this competitive ELISA, optical density is invertly correlated with Kynurenine levels. Only serves as example - do not use for calculation.
DatasheetMSDSProtocol
The L-Kynurenine ELISA is an easy-to-use assay tailored for the quantification of Kynurenine across large series of samples. Intended for research use only, the KYN ELISA features a lower limit of detection at 45.7ng/ml and a minimal sample volume of 20µl, thus allowing longitudinal studies in mice.
| Sample type | Plasma, serum, cell culture supernatant |
| Capacity | 96 tests |
| Sensitivity | < 45.7ng/ml |
| Range | 100 – 10000ng/ml |
| Assay time | Sample acylation 90 min, ELISA overnight |
| Reactivity | Reacts with all species |
| References | Citations in litterature |
Product overview
| Product name | L-Kynurenine ELISA kit |
| Description | Enzyme immunoassay (ELISA) for the quantitative determination of L-Kynurenine in biological fluids and cell supernatant. For research use only |
| Format | 96-well plate |
| Samples | Plasma, serum, cell culture supernatant |
| Minimal sample volume | 20µL |
| Reactivity | Reacts with all species |
| Standard range | 0/100 – 10000ng/ml |
| Sensitivity |
< 47.5ng/ml |
| Specificity | No significant cross-reactivity was observed with L-Kynurenine analogs such as L-Tryptophan, 3-Hydroxy-DL-Kynurenine, 5-Hydroxy-DL-Tryptophan, Tyrosine, Serotonin, Phenylalanin, L-Asparagine and Kynurenic acid |
| Assay time | Sample acylation 90 min and ELISA overnight |
| Storage | Store at 2-8°C for to 6 months |
| Datasheets | Instructions for use, Material Safety Datasheet |
| The kit for in-vitro-diagnostic (IVD) use can be ordered with the catalog no. BA E-2200. |
| Sample collection & storage | Plasma/Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store smaples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | Sample acylation (90 min) |
| ELISA | L-Kynurenine antiserum overnight incubation, revelation and read steps (1h) |
| Detailed protocol | Download instructions for use |
Product citation
- Priming Mesenchymal Stem/Stromal Cells with a Combination of a Low Dose of IFN- and Bortezomib Results in Potent Suppression of Pathogenic Th17 Immunity Through the IDO1-AHR Axis
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Authors : Park et al., Stem Cells
2022-10 - IDO1 is highly expressed in macrophages of patients in advanced tumour stages of oral squamous cell carcinoma
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Authors : Struckmeier et al., Journal of Cancer Reasearch & Clinical Oncology
2022-08 - Kynurenine induces T cell fat catabolism and has limited suppressive effects in vivo
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Authors : Siska et al., eBioMedicine
2021-12 - Cancer-associated fibroblasts educate normal fibroblaststo facilitate cancer cell spreading and T-cell suppression
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Authors : Itoh et al., Molecular Oncology
2021-11 - Circulating concentrations of citrulline, neopterin, kynurenine, and tryptophan during chemoradiation in patients with cervical carcinoma
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Authors : Pospíšková et al., Pteridines
2021-11 - Deep learning model enables the discovery of a novel immunotherapeutic agent regulating the kynurenine pathway
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Authors : Kim et al., Oncoimmunology
2021-11 - Apathy is not associated with a panel of biomarkers in older adults with HIV disease
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Authors : Wood et al., Journal of psychosomatic research
2021-11 - STING activation normalizes the intraperitoneal vascular-immune microenvironment and suppresses peritoneal carcinomatosis of colon cancer
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Authors : Lee et al., Journal of Immunotherapy of Cancer
2021-06 - Decreased IDO1 Dependent Tryptophan Metabolism in Aged Lung during Influenza
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Authors : Jung Cho et al., European Respiratory Journal
2021-05 - IDO1-mediated Trp-kynurenine-AhR signal activation induces stemness and tumor dormancy in oral squamous cell carcinomas
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Authors : Anzai et al., Oral Science International
2021-04 - Effect of repetitive transcranial magnetic stimulation on the kynurenine pathway in stroke patients
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Authors : Masachika et al., Clinical neuroscience
2020-06 - Evaluating the potential role of tryptophan in calf milk replacers to facilitate weaning
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Authors : Yeste et al., Journal of Dairy Science
2020-05 - A highly efficient modality to block the degradation of tryptophan for cancer immunotherapy: locked nucleic acid-modified antisense oligonucleotides to inhibit human indoleamine 2,3-dioxygenase 1/tryptophan 2,3-dioxygenase expression
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Authors : Klar et al., Cancer Immunol Immunother
2020-01 - Physical exercise prevents mice from L-Kynurenine-induced depression-like behavior
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Authors : Su et al., Asian Journal of Psychatry
2019-12 - Adding Indoximod to Hypofractionated Radiotherapy with Anti-PD-1 Checkpoint Blockade Enhances Early NK and CD8 + T-Cell-Dependent Tumor Activity
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Authors : Watanabe et al., Clinical Cancer Research
2019-11 - Lactobacillus maintains healthy gut mucosa by producing L-Ornithine
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Authors : Qi et al., Communications Biology
2019-05 - Exercise training impacts skeletal muscle gene expression related to the kynurenine pathway
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Authors : Allison et al., American Journal of Psycology
2019-01 - Leucine competes with kynurenine for blood-to-brain transport and prevents lipopolysaccharide-induced depression-like behavior in mice
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Authors : Walker et al., Molecular Psychiatry
2018-07 - Chronic Mild Stress Alters Kynurenine Pathways Changing the Glutamate Neurotransmission in Frontal Cortex of Rats
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Authors : Martin-Hernandez et al., Molecular Neurobiology
2018-05 - Use of PD-1 Targeting, Macrophage Infiltration, and IDO Pathway Activation in Sarcomas: A Phase 2 Clinical Trial
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Authors : Toulmonde et al., JAMA oncology
2018-01 - Dysbiosis of the Vaginal Microbiota and Higher Vaginal Kynurenine/Tryptophan Ratio Reveals an Association with Chlamydia trachomatis Genital Infections
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Authors : Ziklo et al., Frontiers in cellular and infection microbiology
2018-01 - In vivo inhibition of tryptophan catabolism reorganizes the tuberculoma and augments immune-mediated control of Mycobacterium tuberculosis
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Authors : Gautam et al., Proceedings of the National Academy of Sciences of the USA
2018-01 - Inhibition of acute lethal pulmonary inflammation by the IDO-AhR pathway
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Authors : Lee et al., Proceedings of the Nation Academy of Sciences of the USA
2017-07 - The peroxyl radical-induced oxidation of Escherichia coli FtsZ and its single tryptophan mutant (Y222W) modifies specific side-chains, generates protein cross-links and affects biological function
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Authors : Escobar-Alvarez et al., Free radical biology & medicine
2017-07 - Biological and clinical significance of tryptophan-catabolizing enzymes in cutaneous T-cell lymphomas
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Authors : Maliniemi et al., Oncoimmunology
2017-02
