Antibodies & immunoassays for small molecules Metanephrine ELISA kit – Research
Urine samples
Ref: BA-E-8400R
CatecholaminesResearch ELISA kits (RUO)ELISA
+Urine Metanephrine ELISA: Standard curve
Standard curve - to be generated every time the assay is performed.
DatasheetMSDSProtocol
This Metanephrine ELISA kit enables to quantify metanephrine in urine samples within 2 hours. Intended for research use only, this quantitative immunoassay demonstrated good correlation with HPLC (see product data sheet).
| Sample type | 25µl urine |
| Samples/kit | 40 samples in duplicate |
| Sensitivity | 13ng/ml |
| Range | 20 – 2 000ng/ml |
| Assay time | Sample hydrolysis and acetylation (45 min), ELISA 1h |
| Reactivity | Reacts with all species |
| Citations | See 2-MET kit |
Product overview
| Product name | Metanephrine ELISA kit – Urine- Fast-Track |
| Description | Competitive ELISA kit for the quantitative measurement of Metanephrine (metadrenaline) levels in urine samples. For research use only |
| Labels | RUO |
| Format | 96-well plate |
| Samples | Urine |
| Sample volume | 25µL |
| Reactivity | Reacts with all species |
| Standard range | 0 / 20 – 2 000ng/ml |
| Sensitivity |
13ng/ml |
| Specificity | No significant cross-reactivity was observed with Metanephrine analogs such as Normetanephrine, 3-methoxytyramine, Adrenaline, Noradrenaline, Dopamine, Vanillic mandelic acid, L-Dopa, Homovanillic acid, L-Tyrosine and Tyramine |
| Assay time | Sample hydrolysis and acetylation 45 min and ELISA 1h |
| Storage | Store at 2-8°C for to 6 months |
| Datasheets | Instructions for use, Material safety datasheet |
| The kit for in-vitro-diagnostic (IVD) use can be ordered with the catalog no. BA E-8400. |
| Sample collection & storage | Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage at -20°C. for long periods (up to 6 months) |
| Sample preparation | Sample hydrolysis (30 min) and acetylation (15min) |
| ELISA | Metanephrine antiserum incubation (30 min), revelation and read steps (30h) |
| Detailed protocol | Download instructions for use |
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