Antibodies & immunoassays for small molecules Serotonin ELISA kit – Research
Serum and Urine samples
Ref: BA-E-8900R
ELISANeuromediatorsTryptophan metabolismResearch ELISA kits (RUO)
+Serotonin ELISA kit
DatasheetMSDSProtocol
This Serotonin (5-HT) ELISA kit is tailored for the rapid measurement of 5-hydroxytryptamine in serum, urine and platelets. Intended for research use only, this fast-track quantitative immunoassay enables the determination of serotonin levels in up to 40 samples within 75 minutes.
| Sample type | 25µl serum, urine |
| Samples/kit | 40 samples in duplicate |
| Sensitivity | 8ng/ml |
| Range | 8 – 2027ng/ml |
| Assay time | Sample acylation (15 min), ELISA (1h) |
| Reactivity | Reacts with all species |
| Citations | 3 |
Product overview
| Product name | Serotonin ELISA kit – Serum, Urine, Platelets – Fast Track |
| Description | Competitive ELISA kit for the quantitative measurement of 5-hydroxytryptamine (5-HT, Serotonin) in serum or urine. For research use only |
| Labels | RUO |
| Format | 96-well plate |
| Samples | 25 µl serum, urine |
| Reactivity | Reacts with all species |
| Standard range | 0 / 8 – 2 170ng/ml |
| Sensitivity |
8ng/ml |
| Specificity | No significant cross-reactivity was observed with Serotonin analogs such as Tryptamine, Melatonin, 5-Hydroxyindole acetic acid, Phenylalanine, Histidine, Tyramine and 5-Hydroxytryptophan |
| Assay time | 1h15 |
| Storage | Store at 2-8°C for up to 6 months |
| Datasheets | Instructions for use, Material safety datasheet |
| The kit for in-vitro-diagnostic (IVD) use can be ordered with the catalog no. BA E-8900 |
| Sample collection & storage | Serum: Do not use haemolytic and especially lipemic samples. Storage: up to 6 hours at 2 – 8°C; for longer periods (up to 6 months) at – 20°C
Urine: Spontaneous or 24-hours urine, collected in a bottle containing 10-15 mL of 6 M HCl. Storage: for longer periods (up to 6 months) at -20°C |
| Sample preparation | Sample acylation (15min) |
| ELISA | Serotonin antiserum incubation (30min), revelation and read steps (30min) |
| Detailed protocol | Download instructions for use |
Product citation
- Dietary Supplementation with Lysine and Threonine Modulates the Performance and Plasma Metabolites of Broiler Chicken
Check the article
Authors : Ishii et al, The journal of poultry science
2019-07 - A novel murine model of severe pulmonary arterial hypertension
Check the article
Authors : Ciuclan et al, American journal of respiratory & clinical care medicine
2011-08 - Effects of delayed laboratory processing on platelet serotonin levels
Check the article
Authors : Sanner et al, Biological research for nursing
2011-08 - Relationship between chronic lead toxicity and plasma neurotransmitters in autistic patients from Saudi Arabia
Check the article
Authors : El-Ansary et al., Clinical biochemistry
2011-07
