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Octopamine Antibody – Rabbit Polyclonal
Ref: IS1033ICCIFIHCNeuromediatorsPolyclonal Antibodies
+Immunolabeling of Octopaminergic and Serotoninergic neurons
Immunostaining of crayfish eyetalk using anti-octopamine rabbit polyclonal antibody (green) and anti-serotonin goat polyclonal antibody (red). Tissus were processed with whole mount protocol of STAINperfect immunostaining kit A. Fluorescent labeled secondary antibodies were used and pictures were acquired by confocal imaging with high magnification.
DatasheetMSDS
The IS1033 anti-Octopamine rabbit polyclonal antibody was validated for immunofluorescence in crayfish brain tissues, using the STAINperfect immunostaining kit A for sample preparation. In combination with the staining kit, the antibody can label Octopamine in cell cultures and tissues for IF/IHC imaging.
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Whole mounts, cell culture, tissue sections & ELISA |
| Staining procedure | STAINperfect immunostaining kit A |
| Format | 50µl (approx. 40 tissue sections) |
Product overview
| Product name | Octopamine antibody – Rabbit pAb |
| Synonyms | Anti-2-Amino-1-(4-hydroxyphenyl)ethanol polyclonal antibody |
| Immunogen | Conjugated Octopamine |
| Specificity | When tested in competitive ELISA, the anti-conjugated Octopamine antibody did not show any significant cross-reactivity with competitors Tyramine, Dopamine or Noradrenaline conjugates |
Storage
| Form | Liquid |
| Purity | Purified anti-serum |
| Storage buffer | Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Material safety datasheet | Download MSDS |
| IF – Cell cultures, Whole mounts, Tissue sections | Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
| Full protocol | Download STAINperfect protocol for Octopamine staining |
| Protocols-at-a-glance |
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| Complete Instructions for Use | Protocol-at-a-glance for cell cultures | Protocol-at-a-glance for whole mounts | Protocol-at-a-glance for tissue sections |
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