The pack combines two independent ELISA kits, one optimized for detecting 3-Hydroxy-Kynurenine and the other for measuring Kynurenic Acid. When used together, they allow calculation of the 3-HK to KYNA ratio, which reflects the balance between neurotoxic and neuroprotective activity within the kynurenine pathway

Both kits are validated for EDTA-plasma and are suitable for use across species, making the pack adaptable to a wide range of translational studies.

Each test requires 50 microliters of plasma. Because the kits are run in parallel, this minimal volume allows researchers to evaluate both metabolites without exhausting precious samples.

The 3-HK kit detects concentrations as low as 0.85 ng/mL with a working range between 2.24 and 87.58 ng/mL. The KYNA kit has a detection limit of 0.53 ng/mL and covers 1.40 to 74 ng/mL.

Each kit is provided in a 96-well plate format, meaning the combined pack offers two full plates—one for 3-HK and one for KYNA—allowing large series or longitudinal studies.

The process involves approximately two and a half hours for sample preparation, an overnight incubation, and about one hour for the detection and reading steps.

Yes, both the 3-HK and KYNA assays have been cross-validated with LC-MS, ensuring their accuracy and reliability.

No, both ELISA kits are species-independent, which makes them suitable for human plasma as well as animal models.

Kits should be kept at 2 to 8 degrees Celsius and remain stable for six months. Plasma samples can be stored short term at 2 to 8 degrees Celsius for up to 48 hours or frozen at minus 20 degrees Celsius or below for long-term preservation.

The ratio of 3-HK to KYNA has been studied in relation to oxidative stress, neuroinflammation, psychiatric conditions, and neurodegenerative diseases. This makes the pack a powerful tool for preclinical and clinical investigations into the kynurenine pathway.

Specificity assessments confirm that no significant cross-reactivity occurs with structurally related compounds, ensuring reliable detection of 3-HK and KYNA.

Researchers purchasing the pack benefit from direct scientific assistance, a return and replacement policy, and troubleshooting help to ensure successful use of both kits.