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IHC validation of 3-HydroxyAnthranilic acid antibody in human brain tissueIHC staining shows accumulation of 3-hydroxy-anthranilic acid in human colon cancer cells, as well as in cells from the tumoral microenvironment. Human paraffin-embedded colon tumor tissue was subjected to pH=6 antigen retrieval, and overnight incubation with primary 3-Hydroxy-Anthranilic acid antibody (1/1000 dilution). After polymer-conjugated secondary antibody exposure, staining was observed through DAB coloration.
3-Hydroxy-Anthranilic acidTryptophan catabolism can be initiated by either indoleamine 2,3 dioxygenase 1 and 2 (IDO1 and IDO2) or the tryptophan 2,3 dioxygenase 2 (TDO2) to produce a series of catabolites collectively known as kynurenines. This pathway has been extensively studied for its immune regulatory functions. The production of 3-hydroxy-Anthranilic acid (3HAA) is thought to play a key role in this phenomenon, with PDK1 being the only molecular target identified. Also, 3HAA has been shown to exert anti-inflammatory effects when administered in an experimental model of multiple sclerosis mice (EAE).
Key features
| Clonality | Monoclonal antibody |
| Host | Mouse |
| Reactivity | Reacts with all species |
| Tested samples | Human tissue sections (brain, lung, tumor) |
| Staining procedure | Perform heat antigen retrieval (pH=6) before standard IHF/IF staining |
| Format | 50µl (approx. 40 tissue sections) |
Frequently asked questions
3-HydroxyAnthranilic acid Antibody – Mouse Monoclonal
The first and only anti-3-HydroxyAnthranilic acid mouse antibody available for research use. This primary mouse monoclonal antibody was validated for IHC in human tumor and brain tissues. When tested by competitive ELISA, the antibody demonstrated strong affinity and high specificity.
€449
92 in stock
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IS001
3-HydroxyAnthranilic acid Antibody – Mouse Monoclonal
3-HydroxyAnthranilic acid mAb – Purified monoclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | 3-Hydroxyanthranilic acid mouse monoclonal antibody |
| Synonyms | Anti-3-Hydroxy-Anthranilic acid antibody
2-Amino-3-hydroxybenzoic acid antibody 3-OH-Anthranilic acid antibody 3-hydroxanthranilate antibody 3-OHAA antibody |
| Immunogen | Conjugated 3-Hydroxyanthranilic acid |
| Isotype |
IgG1 k chain |
| Clone |
Clone 5B2-G2 |
| Specificity | When tested in competitive ELISA, the anti- 3-HydroxyAnthranilic acid antibody did not show any significant cross reactivity with Anthranilic acid or Cinnabarinic acid conjugates |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified IgG |
Instructions
| Storage |
Store at +4°C for short term (6 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunohistochemistry (IHC) | Dilute at 1:200-1:2000. Perform heat antigen retrieval (pH=6) before initiating IHC staining protocol on paraffin-embedded and frozen sections |
| Immunofluorescence (IF) |
Dilute at 1:100-1:1000 on paraffin-embedded and frozen sections. Perform heat antigen retrieval and incubate with fluorescent dyes conjugated secondary antibody |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
