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Whole mount hindbrain immunostaining of 3MT containing cells.Anti-3-Metoxytyramine antibody highlights the soma and projections of 3MT positive neurons in the hindbarin of mouse E14.5 embryo. Staining was performed using STAINperfect immunostaining kit A, following the protocol for whole mounts. Secondary antibody (goat anti-rabbit Alexa Fluor® 488) was used and picture was acquired by confocal imaging at high magnification.
3-MT spinal cord immunostaining of whole-mount mouse embryosAnti-3-Metoxytyramine antibody highlights the fibers of 3MT neurons in the spinal cord of mouse E14.5 embryo. Staining was performed with STAINperfect immunostaining kit A, following the protocol for whole mounts. Secondary antibody was used and picture was acquired by confocal imaging at high magnification.
3-Methoxytyramine neuronal staining in free-floating brain sectionsAnti-3-Metoxytyramine antibody highlights the presence of 3MT neurons in the midbrain of adult mouse with granular pattern. Staining was performed using STAINperfect immunostaining kit A, on free floated cryosections following the protocol for whole mount samples. Fluorescent labelled secondary antibody was used and picture was acquired by confocal imaging at high magnification.
3-MT immunostaining of adult mouse cerebellumAnti-3-Metoxytyramine antibody highlights the soma and projections of 3MT positive neurons in the hindbarin of mouse E14.5 embryo. Staining was performed using STAINperfect immunostaining kit A, following the protocol for whole mounts. Secondary antibody (goat anti-rabbit Alexa Fluor® 488) was used and picture was acquired by confocal imaging at high magnification.
Affinity & specificity of anti-3-Methoxy-Tyramine antibodyCompetitive ELISA demonstrates that low amounts of 3-Methoxy-Tyramine conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of 3-Methoxy-Tyramine competitors Tyramine or Dopamine do not affect reaction (high specificity).
3-Methoxytyramine (3-MT)3-Methoxytyramine is a dopamine metabolite synthesized by the enzyme catechol-O-methyltransferase. Together with catecholamines, increased 3-MT urinary excretion is used as a biomarker for biochemically active head and neck paragangliomas. Considered for a long time as a physiologically inactive metabolite, 3-MT was recently shown to induce behavioral effects through the trace amine associated receptor 1 (TAAR1).
Key features
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Mouse brain tissues |
| Staining procedure | STAINperfect immunostaining kit A (protocols for cell cultures, whole mounts, tissue sections) |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
3-Methoxytyramine Antibody – Rabbit Polyclonal
The anti-3-Methoxytyramine (3-MT) antibody IS1019 is a rabbit polyclonal antibody validated for IF in mouse brain tissues (whole mounts, free-floating sections). In samples prepared with the STAINperfect immunostaining kit A, this antibody allows direct 3MT visualization in whole mounts, cell cultures and tissue sections.
€449
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IS1019
3-Methoxytyramine Antibody – Rabbit Polyclonal
Anti-3-Methoxytyramine (3-MT) pAb – Purified polyclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | 3-Methoxytyramine antibody |
| Synonyms | Anti-3-Methoxytyramine polyclonal antibody3-MT polyclonal antibody |
| Immunogen | Conjugated 3-Methoxytyramine |
| Specificity | When tested in competitive ELISA, the anti-conjugated 3-Methoxytyramine antibody do not show significant cross-reactivity with several competitors including Tyramine and Dopamine conjugates |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified anti-serum |
Instructions
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunofluorescence (IF) |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample (Cell cultures, Whole mounts, Tissue sections) |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
