The clear path to small molecule visualization & quantification

Key features

Sample type Plasma, serum
Capacity 2 x 96 tests
Sensitivity ARG: 2.1µM
CIT: 1.47µM
Range ARG: 5.12 – 200µM
CIT: 6.4 – 250µM
Assay time Sample preparation (3h), ELISA overnight
Reactivity Reacts with all species
Application Research use only

Citations

Aucune citation disponible.

Frequently asked questions

ISE-0510R

Citrulline-to-Arginine ratio ELISA pack

Measuring the Citrulline-to-Arginine ratio is indicative of nitric oxide synthase (NOS) activity, which converts Arginine into Citrulline and Nitric Oxide (NO). A decreased plasma CIT-to-ARG ratio thus suggests high NO production, and may serve as a biomarker in metabolic and cardiovascular conditions.

10491198

Number of citations :

93 in stock

93 in stock

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ISE-0510R

Citrulline-to-Arginine ratio ELISA pack

ELISA pack composed of :

– IS-I-0400R – Arginine ELISA kit – Research test for the detection of L-Arginine
– IS-I-1500R – Citrulline ELISA kit – Research test for the detection of L-Citrulline

Specifications

Product name Citrulline-to-Arginine ELISA pack
Description Two enzyme immunoassays (ELISA) allowing the quantitative determination of L-Citrulline and L-Arginine in plasma/serum samples. For research use only
Format 2 x 96-well plates
Samples Plasma, serum
Minimal sample volume 20µL
Reactivity Reacts with all species
Standard range ARG: 5.12 – 200µM
CIT: 6.4 – 250µM
Sensitivity ARG: 2.1µM
CIT: 1.47µM
Specificity No significant cross-reactivity was observed with analogs for each kit. See product pages for Arginine ELISA and Citrulline ELISA
Assay time Sample preparation (3h), ELISA overnight

Instructions

Storage  Store at 2-8°C for to 6 months
Sample collection & storage Plasma: Use EDTA as anticoagulantSerum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands.

Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months)

Sample preparation Sample preparation (3h)
ELISA Antisera overnight incubation, revelation and read steps (1h)

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