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Affinity & specificity of anti-D-Glutamate antibodyCompetitive ELISA demonstrates that low amount of D-Glutamate conjugate is required to abolish antigen-antibody reaction (high affinity), while rising concentrations of L-Glutamate and D-Aspartate conjugates do not affect reaction (high specificity).
D-Glutamate detection in primary mouse cortical neuronsPrimary mouse cortical neurons were stained using ImmuSmol anti-D-Glutamate antibody using the STAINperfect immunostaining kit A and following the optimized protocol for cell culture. Alexa fluor 488 conjugated secondary antibodies were used and image was captured by confocal imaging.
Key features
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Rodent primary neurons |
| Staining procedure | STAINperfect immunostaining kit A (protocols for cell cultures, whole mounts, tissue sections) |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
D-Glutamate Antibody – Rabbit Polyclonal
IS1002 is the only anti-D-Glutamate antibody available to date. This primary rabbit polyclonal antibody allows to stain for D-glutamic acid in cell cultures, whole mounts and tissue section when used in combination with the STAINperfect immunostaining kit A. Our team validated this antibody in mouse primary cortical neurons.
€449
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IS1002
D-Glutamate Antibody – Rabbit Polyclonal
Anti-D-Glutamate pAb – Purified polyclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | D-Glutamate antibody – Rabbit pAb |
| Synonyms | Anti-D-Glutamic acid antibody |
| Immunogen | Conjugated D-Glutamate |
| Specificity | When tested in competitive ELISA, the anti-conjugated D-Glutamate antibody did not show any significant cross reactivity with D-Glutamate analogs, L-Glutamate and D-Aspartate |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified anti-serum |
Instructions
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunofluorescence (IF) |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample (Cell cultures, Whole mounts, Tissue sections) |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
