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GABA and Dopamine immunostaining in crayfish eyetalkUsing STAINperfect immunostaining kit A, the anti-GABA (red)chicken polyclonal antibody (IS1036) combined with anti-dopamine (green) rabbit polyclonal antibody (IS1005) demonstrate the presence of GABA and dopamine in crayfish eyetalk. The staining was performed according to the protocol optimized for whole-mount samples. Fluorescent labeled secondary antibodies were used and image obtained by fluorescence imaging.
GABA and L-Serine in mouse primary neurons culturePrimary mouse cortical neurons were stained using anti-GABA chicken polyclonal antibody (red) and anti-L-Serine polyclonal rabbit antibody (green) using the STAINperfect immunostaining kit A and according to the optimized protocol for cell culture. Co-immunostaining appears in yellow. Alexa fluor 546 and 488 conjugated secondary antibodies respectively were used and picture obtained by confocal imaging.
GABA immunostaining in mouse cortical primary neuronsPrimary mouse cortical neurons were stained using anti-GABA chicken polyclonal antibody and the STAINperfect immunostaining kit A according to the optimized protocol for cell culture. Alexa fluor 546 conjugated secondary antibody was used and picture obtained by confocal imaging.
Affinity & specificity of anti-GABA antibodyCompetitive ELISA demonstrates that low amounts of GABA conjugate are required to abolish antigen-antibody reaction (high affinity), while rising concentrations of GABA competitors beta Alanine or D-Alanine do not affect reaction (high specificity).
Gamma-aminobutyric acid (GABA)In the mammalian brain, inhibitory neurotransmitter Gamma-aminobutyric acid (GABA) is mainly synthesized from excitatory L-Glutamate by enzyme glutamic acid decarboxylase (GAD). Regulating neuronal excitability, GABAergic synapses are present throughout the CNS, although GABA is found most highly concentrated in the subtantia nigra, the globus pallidus nuclei, the hypothalamus, the periaqueductal grey matter and the hyppocampus.
Key features
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Mouse primary neurons, Human iPS-derived neurons, and crayfish & rodent brain tissues |
| Staining procedure | STAINperfect immunostaining kit A (protocols for cell cultures, whole mounts, tissue sections) |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
GABA Antibody – Chicken Polyclonal
Our team validated the chicken polyclonal anti-GABA antibody IS1036 for immunofluorescence (IF) in mouse primary neurons and crayfish tissues using the STAINperfect immunostaining kit A. This antibody supports multiplex staining with rabbit polyclonal or mouse monoclonal antibodies. In recent papers, it allowed specific visualization of gamma-aminobutyric acid (GABA) in human iPSC-derived neurons, mouse primary neurons, and mouse brain tissues.
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For questions, protocol recommendations, and troubleshooting, you have direct access to the scientist that developed the antibody or ELISA kit you’re using.
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IS1036
GABA Antibody – Chicken Polyclonal
Anti-GABA pAb – Purified polyclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | GABA antibody – Chicken pAb |
| Synonyms | Anti-gamma-Aminobutyric acid antibody |
| Immunogen | Conjugated GABA |
| Specificity | When tested in competitive ELISA, the anti-conjugated GABA antibody did not show any significant cross reactivity with gamma-Aminobutyric acid analogs, including beta-Alanine and D-Alanine |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified anti-serum |
Instructions
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunofluorescence (IF) |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample (Cell cultures, Whole mounts, Tissue sections) |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
