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GABA labeling (green) in mouse cortical primary neuronsImmunodetection of GABA- (green) and MAP2- (red) positive neurons in mouse primary cortical culture. GABAergic neurons were stained using IS1006 anti-GABA antibody combined with MAP2 antibody using the stainperfect immunostaining kit A and appropriate protocol, respectively. Alexa fluor 488 and 546 secondary antibodies were used and pictures were captured by high-content imaging.
GABAergic neuronal networkin a mature rat cortical culture revealed through GABA immunoreactivity detection using either our rabbit polyclonal GABA antibody (#IS1006) with the STAINperfect immunostaining kit A.
L-Glutamate and GABA in adult mouse primary cortical neuronsAdult mouse primary cortical neurons were stained with mouse monoclonal anti-L-glutamate antibody (red) combined with anti-GABA rabbit polyclonal antibody (green). Staining was performed using optimized sample preparation with STAINperfect immunostaining kit A and according to the protocol for cell culture. Fluorescent labeled secondary antibody were used and pictures were acquired by confocal imaging.
GABA labeling (green) in rat cortical primary neuronsImmunodetection of GABA- (green) and MAP2- (red) positive neurons in rat primary cortical culture. Total and GABAergic neurons were stained using an anti-MAP2 antibody and IS anti-GABA antibody combined with the stainperfect immunostaining kit A and appropriate protocol, respectively. Alexa fluor 546 and 488 secondary antibodies were used and pictures were captured by high-content imaging.
Gamma-aminobutyric acid (GABA)In the mammalian brain, inhibitory neurotransmitter Gamma-aminobutyric acid (GABA) is mainly synthesized from excitatory L-Glutamate by enzyme glutamic acid decarboxylase (GAD). Regulating neuronal excitability, GABAergic synapses are present throughout the CNS, although GABA is found most highly concentrated in the subtantia nigra, the globus pallidus nuclei, the hypothalamus, the periaqueductal grey matter and the hyppocampus.
Key features
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Rodent primary neurons, Rabbit Brain Tissues, Human iPSC-derived neurons |
| Staining procedure | STAINperfect immunostaining kit A (protocols for cell cultures, whole mounts, tissue sections) |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
GABA Antibody – Rabbit Polyclonal
The anti-GABA antibody IS1006 has been validated by our team for immunofluorescence in primary mouse and rat neurons using the STAINperfect immunostaining kit A. Additionally, recent scientific studies have employed this antibody to highlight GABAergic systems in human stem cell-derived neurons and rabbit olfactory bulb tissues. When combined with the STAINperfect immunostaining kit A, this rabbit polyclonal antibody enables the clear visualization of gamma-aminobutyric acid (GABA) in cell cultures, tissue sections, and whole mounts from any species.
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For questions, protocol recommendations, and troubleshooting, you have direct access to the scientist that developed the antibody or ELISA kit you’re using.
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A knowledge base containing protocols, Frequently Asked Questions, and troubleshooting tips is also available online.
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IS1006
GABA Antibody – Rabbit Polyclonal
Anti-GABA pAb – Purified polyclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | GABA antibody – Rabbit Polyclonal antibody |
| Synonyms | Anti-Gamma-Aminobutyric acid antibody |
| Immunogen | Conjugated GABA |
| Specificity | When tested in competitive ELISA, the anti-conjugated GABA antibody did not show any significant cross reactivity with Gamma-Aminobutyric acid analogs, including Beta-Alanine and D-Alanine |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified anti-serum |
Instructions
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunofluorescence (IF) |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample (Cell cultures, Whole mounts, Tissue sections) |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
