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Cross-validation of L-Arginine, L-Ornithine, and L-Citrulline ELISA and LC/MS data in human plasma samplesArg, Cit and Orn were quantified in human plasma samples from healthy subjects using respectively IS-0400R, IS-I-1500R and IS-I-1000R ELISA kits, or by LC/MS. Correlation study confirmed the accuracy of the immunoassays.
Key features
| Sample type | Plasma, serum |
| Capacity | 3 x 96 tests |
| Sensitivity | ARG: 2.1µM ORN: 8.5µM CIT: 1.47µM |
| Range | ARG: 5.12 – 200µM ORN: 20 – 781.25µM CIT: 6.4 – 250µM |
| Assay time | Sample preparation (3h), ELISA overnight |
| Reactivity | Reacts with all species |
| Application | Research use only |
Frequently asked questions
Global Arginine Bioavailability ratio (GABR) ELISA pack
This ELISA pack provides an easy way to assess the plasma/serum Global Arginine Bioavailability Ratio (GABR), defined as arginine/[ornithine + citrulline]. The GABR indicates the availability of arginine for Nitric Oxide production. Low GABR may serve to assess endothelial dysfunction, atherosclerosis, and cardiovascular disorders.
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ISE-451000R
Global Arginine Bioavailability ratio (GABR) ELISA pack
ELISA pack composed of :
– IS-I-0400R – Arginine ELISA kit – Research test for the detection of L-Arginine
– IS-I-1000R- Ornithine ELISA kit – Research test for the detectionb of Ornithine
– IS-I-1500R – Citrulline ELISA kit – Research test for the detection of L-Citrulline
Specifications
| Product name | Global arginine bioavailability ratio (GABR) ELISA pack |
| Description | Three enzyme immunoassays (ELISA) allowing the quantitative determination of L-Arginine, L-Ornithine and L-Citrulline in plasma/serum samples. For research use only |
| Format | 3 x 96-well plates |
| Samples | Plasma, serum |
| Minimal sample volume | 20µL |
| Reactivity | Reacts with all species |
| Standard range | ARG: 5.12 – 200µM ORN: 20 – 781.25µM CIT: 6.4 – 250µM |
| Sensitivity | ARG: 2.1µM ORN: 8.5µM CIT: 1.47µM |
| Specificity | No significant cross-reactivity was observed with analogs for each kit. See product pages for Arginine ELISA, Ornithine ELISA, and Citrulline ELISA |
| Assay time | Sample preparation (3h), ELISA overnight |
Instructions
| Storage | Store at 2-8°C for to 6 months |
| Sample collection & storage | Plasma: Use EDTA as anticoagulant Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands.Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | Sample preparation (3h) |
| ELISA | Antisera overnight incubation, revelation and read steps (1h) |
