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Glycine detection in primary mouse cortical neuronsPrimary mouse cortical neurons were stained using IS1034 anti-Glycine antibody using the STAINperfect immunostaining kit A and according to the optimized protocol for cell culture. Alexa fluor 488 conjugated secondary antibody was used and picture obtained by confocal imaging.
Glycine detection in primary mouse cortical neurons – fibers and somaPrimary mouse cortical neurons were stained with IS1034 anti-Glycine antibody using the STAINperfect immunostaining kit A and according to the optimized protocol for cell culture. After incubation with secondary antibody, this staining reveals the presence of Glycine within fibers and soma of some neurons. Image was obtained by confocal imaging.
Key features
| Clonality | Polyclonal antibody |
| Host | Rabbit |
| Reactivity | Reacts with all species |
| Tested samples | Mouse primary neurons |
| Staining procedure | STAINperfect immunostaining kit A (protocols for cell cultures, whole mounts, tissue sections) |
| Format | 50µL (approx. 40 tissue sections) |
Frequently asked questions
What is the IS1034 glycine antibody used for?
The rabbit polyclonal anti-glycine antibody is developed for the detection of glycine in biological samples. It enables the study of glycinergic systems in cell cultures, whole mounts, and tissue sections.
How was the IS1034 validated?
This antibody was validated for immunofluorescence in mouse primary cortical neurons. Validation was performed using the STAINperfect immunostaining kit A to ensure specific and reliable detection.
Can this primary antibody be combined with other antibodies?
Yes, the antibody is compatible with multiplex immunostaining workflows, allowing researchers to combine glycine detection with other neuronal or neurotransmitter markers in the same sample.
What applications are supported by the glycine antibody?
The glycine antibody supports immunofluorescence studies in cell cultures, tissue sections, and whole mounts. It has been developed to provide specific labeling of glycine within neuronal systems.
Is the IS1034 pAb cross-species compatible?
Although validated in mouse primary cortical neurons, the glycine antibody can be applied to samples from different species when combined with the STAINperfect immunostaining kit A, expanding its use in comparative neuroscience research.
Why choose a rabbit polyclonal antibody?
Rabbit polyclonal antibodies recognize multiple epitopes of glycine, which improves sensitivity and signal detection in immunofluorescence experiments. This makes them particularly suitable for studying complex glycinergic networks.
Glycine Antibody – Rabbit Polyclonal
This rabbit polyclonal anti-glycine antibody was validated for immunofluorescence (IF) in mouse primary cortical neurons using the STAINperfect immunostaining kit A. When combined with the kit, this antibody enables direct visualization of glycine in cell cultures, whole mounts, and tissue sections.
€ 449
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IS1034
Glycine Antibody – Rabbit Polyclonal
Anti-Glycine pAb – Purified polyclonal antibody for research use only (liquid 50µL)
Specifications
| Product name | Glycine antibody – Rabbit polyclonal Ab |
| Synonyms | Anti-aminoacetic acid antibody |
| Immunogen | Conjugated Glycine |
| Specificity | When tested in competitive ELISA, the anti-conjugated Glycine antibody did not show any significant cross reactivity with Glycine analogs, including Alanine and Serine conjugates |
| Volume | 50 µL |
| Form | Liquid |
| Purity | Purified anti-serum |
Instructions
| Storage |
Store at +4°C for short term (1-2 months). Aliquot and store at -20°C for long term. Avoid repeated freeze / thaw cycles |
| Immunofluorescence (IF) |
Dilute antibody with the antibody diluent provided in the STAINperfect immunostaining kit A. Use at 1/250 -1/1000 dilution. Follow the STAINperfect protocol suited to your sample (Cell cultures, Whole mounts, Tissue sections) |
| Comments | Optimal working dilutions must be determined by the end-user |
| Restrictions | For research use only |
