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Histamine ELISA kit Ultra-Sensitive – Any sample BA-E-5800R

Histamine ELISA: Standard curveStandard curve - to be generated every time the assay is performed.

Key features

Sample type	Any sample
Capacity	96-well plate
Sensitivity	0.2 ng/mL
Range	0.5 – 50 ng/mL
Assay time	Sample acylation (90 min), incubation (3h or overnight), ELISA revelation & read steps (90 min)
Reactivity	Reacts with all species
Application	Research use only

Citations

2025 Arifuku et al., - Establishment of a Murine Desensitization Model to PLA2-induced Anaphylaxis, Dokkyo Medical Journal, Species : Mouse ; Sample type : Plasma ;
2025 Kurita et al., - Establishment of a novel human basophil cell line for functional analysis and in vitro allergy testing, Allergology International, Species : Human ; Sample type : Cell culture supernatant ;
2023 Komuro et al., - Ganglioside GM3 deficiency enhances mast cell sensitivity, The FEBS Journal, Species : Human ; Sample type : Cell culture supernatant ;
2023 Riedl et al., - Establishment and Characterization of Mild Atopic Dermatitis in the DNCB-Induced Mouse Model, International Journal of Molecular Sciences, Species : Mouse ; Sample type : Plasma ;
2019 von Beek et al., - Streptococcal sagA activates a proinflammatory response in mast cells by a sublytic mechanism, Cellular Microbiology, Species : Human ; Sample type : Cell culture ;
2017 Heidinger et al., - Decreased viability and proliferation of CHANG conjunctival epithelial cells after contact with ultraviolet light-irradiated pollen, Cutaneous and Ocular Toxicology, Species : Human ; Sample type : Pollen ;

Frequently asked questions

BA-E-5800R

Histamine ELISA kit – Any sample

This research Histamine ELISA kit enables the ultra-sensitive measurement of histamine in any biological sample: tissue homogenates, cell culture supernatants, body fluids, etc. With a minimal sample volume as low as 10µl, this histamine immunoassay allows longitudinal studies in mice and rat models.

€599

Number of citations :

100 in stock

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Direct Customer Support

For questions, protocol recommendations, and troubleshooting, you have direct access to the scientist that developed the antibody or ELISA kit you’re using.

Direct customer support is available all business days.

A knowledge base containing protocols, Frequently Asked Questions, and troubleshooting tips is also available online.

They published with this product

Institute of Nutritional Science, Friedrich Schiller University

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BA-E-5800R

Histamine ELISA kit – Any sample

Histamine ELISA kit – Ultra-sensitive research test for the detection of histamine

Specifications

Product name	Histamine ELISA kit – Any sample – Research
Description	Enzyme immunoassay (ELISA) for the quantitative determination of histamine levels in biological samples. Research use only
Format	96-well plate
Samples	Any sample, including homogenates
Minimal sample volume	10 µL
Reactivity	Reacts with all species
Standard range	0 / 0.5 – 50 ng/mL
Sensitivity	0.2 ng/mL
Specificity	No significant cross-reactivity was observed with Histamine analogs such as 3-Methyl-Histamine, Tyramine, L-Phenylalanine, L-Histidine, L-Tyrosine, Tryptamine, 5-Hydroxy-Indole-Acetic Acid, Serotonin.
Assay time	Sample acetylation (90 min) and ELISA overnight

Instructions

Storage	Store at 2-8°C for to 6 months
Sample collection & storage	See instructions for use
Sample preparation	Sample predilution and acylation (1h30)
ELISA	Histamine antiserum incubation (3h or overnight), revelation and read steps (1h30)