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Cross-validation of Homoarginine ELISA and LC/MS data in human plasma sampleshArg was quantified in human plasma samples from 40 healthy subjects either using IS-I-0500 ELISA kit or by liquid chromatography–mass spectrometry (LC/MS). Correlation coefficient of 0.964 confirms the accuracy of the immunoassay.
Key features
| Sample type | Plasma, serum |
| Capacity | 96-well plate |
| Sensitivity | 125nM |
| Range | 0.32 – 12.5µM |
| Assay time | Sample preparation 3h, ELISA overnight |
| Reactivity | Reacts with all species |
| Application | Research use only |
Frequently asked questions
HomoArginine ELISA kit
The HomoArginine (hArg) ELISA kit enables sensitive and quantitative measurement of the non-essential amino acid HomoArginine in serum and plasma samples. Validated in human plasma with strong concordance to LC-MS results, this user-friendly assay provides robust performance across a range of study designs. Species-independent and requiring only 20 µL of sample, it is ideally suited for both preclinical and clinical research applications.
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IS-I-0500R
HomoArginine ELISA kit
HomoArginine ELISA kit – Research test for the detection of HomoArginine (hARG)
Specifications
| Product name | HomoArginine ELISA kit |
| Description | Competitive ELISA kit for the quantitative measurement of Homoarginine (hARG) in serum and plasma samples. For research use only |
| Format | 96-well plate |
| Samples | Plasma, serum |
| Minimal sample volume | 250µL |
| Reactivity | Reacts with all species |
| Standard range | 0.32 – 12.5µM |
| Sensitivity | 125nM |
| Specificity | No significant cross-reactivity was observed with L-Homoarginine |
| Assay time | Sample preparation 3h and ELISA overnight |
Instructions
| Storage | Store at 2-8°C for to 6 months |
| Sample collection & storage | Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strandsStore samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | Sample preparation (3 hours) |
| ELISA | Homoarginine antiserum overnight incubation, revelation and read steps (1h) |
