The KMO Activity ELISA Pack (3-HK/KYN ratio) combines two ELISA kits designed to quantify 3-Hydroxy-Kynurenine (3-HK) and L-Kynurenine (KYN) in plasma. By measuring both metabolites, researchers can calculate the 3-HK/KYN ratio as a direct readout of KMO enzymatic activity, supporting studies in tryptophan metabolism.

The pack is validated for EDTA plasma samples. A minimal volume of 50 µL assay is required to perform the assays.

The pack contains two 96-well plates, one dedicated to 3-HK and one to KYN. The limit of detection is around 0.8 ng/mL for 3-HK and 47.5 ng/mL for KYN. The dynamic range is 2 to 88 ng/mL for 3-HK and 100 to 10,000 ng/mL for KYN.

Sample preparation typically requires about two and a half hours. The ELISA procedure includes an overnight incubation step followed by colorimetric revelation and plate reading, which takes about one additional hour.

Yes, the ELISA pack reacts with all species, making it suitable for both preclinical and clinical research studies.

The kit should be stored refrigerated between 2 and 8 °C for up to six months. Plasma samples can be kept at 2 to 8 °C for up to 48 hours or frozen at -20 °C for storage up to six months.

No significant cross-reactivity with structural analogs has been reported. Each assay is validated for high specificity toward its respective target, 3-HK or KYN.

The pack is intended for research use only and is not approved for diagnostic or clinical applications. It is most relevant for pharmacodynamic studies, preclinical drug development, and investigations of metabolic or inflammatory pathways.

Each kit includes a 96-well plate for 3-HK and a 96-well plate for KYN, allowing up to 48 tests in duplicate per metabolite.

The product is supplied with detailed protocols and datasheets. Technical support is available to help with assay setup, optimization, and troubleshooting.

The ELISA pack is shipped worldwide (room temperature). Returns are accepted within 30 days if ordered by mistake, and replacements or refunds are available in case of product damage or non-conformity.

The KMO Activity ELISA Pack is used to study kynurenine monooxygenase activity in contexts such as neuroinflammation, neurodegenerative disease, immuno-oncology, and metabolic disorders. It is particularly useful for monitoring pharmacodynamic effects of KMO inhibitors and for understanding the balance of tryptophan metabolites in health and disease.