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KYN / KYNA / QA ELISA pack – Standard curvesExamples of standard curves obtained with the Kynurenine, Kynurenic acid, and Quinolinic acid ELISA kits. In these competitive ELISAs, optical density is inversely correlated with Kynurenine/Kynurenic acid/Quinolinic acid levels. For illustration purposes only – do not use for calculations.
KYN, KYNA and QA along the Kynurenine PathwayKynurenine (KYN), Kynurenic acid (KYNA) and Quinolinic acid (QA) are downstream metabolites produced along the Kynurenine Pathway well-known to possess several neuromodulatory functions, as well as immuno-modulatory properties. Measurement of KYNA and QA in biological samples has been presented as possible biomarkers in several diseases.
Key features
| Sample type | Plasma, Serum |
| Capacity | 3×96 tests |
| Sensitivity | LoD KYN: 47.5ng/mL LoD KYNA: 0.53ng/mL LoD QA: 6ng/mL |
| Range | KYN: 100 – 10000ng/ml KYNA: 1.40 – 74ng/ml QA: 25 – 2000ng/ml |
| Assay time | Sample acetylation (90-120 min), overnight incubation, ELISA revelation & read step (1h) |
| Reactivity | Reacts with all species |
| Application | Research use only |
Frequently asked questions
Kynurenine/Kynurenic Acid/Quinolinic Acid ELISA pack (3-KYNs ELISA pack)
The Kynurenine / Kynurenic Acid / Quinolinic Acid ELISA Pack delivers rapid, quantitative insight into tryptophan catabolism across large sample sets. Supplied as three ready-to-use kits for research use only, it combines picogram-level sensitivity with minimal sample requirements (25–50 µL), making it ideal for both pre-clinical and clinical research studies.
€1499€1797
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ISE-220102R
Kynurenine/Kynurenic Acid/Quinolinic Acid ELISA pack (3-KYNs ELISA pack)
ELISA pack composed of :
- BA-E-2200 – Kynurenine ELISA kit – Research test for the detection of L-Kynurenine
- IS-I-0200 – Kynurenic acid ELISA kit – Research test for the detection of Quinolinic acid
- IS-I-0100 – Quinolinic acid ELISA kit – Research test for the detection of Quinolinic acid
Specifications
| Product name | Kynurenine/Kynurenic Acid/Quinolinic Acid ELISA pack |
| Description | Three enzyme immunoassays (ELISA) allowing the quantitative determination of L-Kynurenine, Kynurenic acid and Quinolinic acid in serum samples. For research use only |
| Format | 3x 96-well plate |
| Samples | Plasma, Serum |
| Minimal sample volume | 20-50µL |
| Reactivity | Reacts with all species |
| Standard range | KYN: 100 – 10000ng/mL KYNA: 1.40 – 74ng/mL QA: 200 – 2000µg/mL |
| Sensitivity |
LoD KYN: 47.5ng/mL |
| Specificity | No significant cross-reactivity was observed with analogs for each kit. See product pages for L-Kynurenine ELISA, Kynurenic acid ELISA, and Quinolinic acid ELISA |
| Assay time | Sample acetylation (90-120 min), overnight incubation, ELISA revelation & read step (1h) |
Instructions
| Storage | Store at 2-8°C for to 6 months |
| Sample collection & storage | Serum: Do not use lipemic, haemolytic samples, as well as samples containing precipitates or fibrin strands. Store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | KYN: Sample acylation (90 min) KYNA: Sample derivatization (90 min) QA: Sample acylation (120 min) |
| ELISA | Antisera overnight incubation, revelation and read steps (1h) |
