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L-Glutamine ELISA kit – High Sensitivity – Plasma, Serum, Supernatant IS-I-1100RValidated IS-I-1100 ELISA kit allows for the determination of L-Glutamine (Gln) in plasma samples of a volume as low as 10μL and with a sensitivity of 3µM.
Tumor cell lines from both murine and human origins degrade Glutamine to support their high proliferating rate in a Gls1 dependent manner.Cancer cell lines were seeded and were then left untreated or exposed to a specific Gls1 inhibitor (CB-839 at 1µM). At the end of treatment duration, culture supernatants were collected and processed for Glutamine quantitation by ELISA (#IS-I-1100). As depicted here, the different tumor cell lines harbored a variable Glutamine consumption rate - being higher in murine than in human tumor cell lines. Among the different human cell lines, a higher rate of Glutamine degradation is observed for colorectal (HT29 & HCT116) followed by lung (H1299 & A549) and then breast (BT-549 & MDA-MB231) cancer cell lines. Also, it’s noteworthy that Gls1 blockade by CB-839 only partially reversed Glutamine consumption, thus indicating the participation of another catabolic pathway, more presumably Gls2.
Cross-validation of Glutamine ELISA and LC/MS data in human plasma samplesGln was quantified in human plasma samples from 40 healthy subjects using IS-I-1100 ELISA kit or by LC/MS. Correlation study showed R2=0.9946, thereby confirming the accuracy of the immunoassay.
Key features
| Sample type | Plasma, serum, cell culture supernatant |
| Capacity | 96-well plate |
| Sensitivity | 3µM |
| Range | 21 – 3125µM |
| Assay time | Sample preparation 3h, ELISA overnight |
| Reactivity | Reacts with all species |
| Application | Research use only |
Frequently asked questions
L-Glutamine ELISA kit
The IS-I-1100R Glutamine ELISA kit enables the sensitive and quantitative determination of L-Glutamine (Gln) in plasma, serum, and cell culture supernatants. Cross-validated against LC-MS in human plasma samples, the assay has also been successfully applied to both human and mouse cell culture models (see supporting data). Species-independent and requiring as little as 10 µL of sample, the kit combines high sensitivity with ease of use, making it an ideal tool for both preclinical research and clinical studies.
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IS-I-1100R
L-Glutamine ELISA kit
L-Glutamine ELISA kit – Research test for the detection of L-Glutamine (Gln)
Specifications
| Product name | Glutamine ELISA kit |
| Description | Competitive ELISA kit for the quantitative measurement of L-Glutamine (Gln) in plasma and serum samples. For research use only |
| Format | 96-well plate |
| Samples | Plasma, serum, cell culture supernatant |
| Minimal sample volume | 10µL |
| Reactivity | Reacts with all species |
| Standard range | 21 – 3125µM |
| Sensitivity |
3µM |
| Specificity | No significant cross-reactivity was observed with Ornithine, Arginine, Asparagine, D-Glutamine and L-Glutamic acid |
| Assay time | Sample preparation 3h and ELISA overnight |
Instructions
| Storage | Store at 2-8°C for to 6 months |
| Sample collection & storage | EDTA Plasma – store samples at 2-8°C for up to 48h or -20°C for longer period (up to 6 months) |
| Sample preparation | Sample preparation (3 hours) |
| ELISA | L-Glutamine antiserum overnight incubation, revelation and read steps (1h) |
